Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.3 extracted from

  • Wang, Y.; Xiao, D.; Liu, Q.; Zhang, Y.; Hu, C.; Sun, J.; Yang, C.; Xu, P.; Ma, C.; Gao, C.
    Two NAD-independent l-lactate dehydrogenases drive L-lactate utilization in Pseudomonas aeruginosa PAO1 (2018), Environ. Microbiol. Rep., 10, 569-575 .
    View publication on PubMed

Application

Application Comment Organism
medicine both LldD and LldA are important for growth of Pseudomonas aeruginosa PAO1 in synthetic cystic fibrosis sputum medium Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.336
-
L-lactate pH 7.4, temperature not specified in the publication Pseudomonas aeruginosa
1.102
-
L-lactate pH 7.4, temperature not specified in the publication Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Pseudomonas aeruginosa 16020
-

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HV37
-
-
Pseudomonas aeruginosa Q9I197
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + 2 ferricytochrome c
-
Pseudomonas aeruginosa pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Synonyms

Synonyms Comment Organism
lldA
-
Pseudomonas aeruginosa
LldD
-
Pseudomonas aeruginosa
PA4771
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FMN
-
Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function disruption of the lldD gene impairs the growth of this strain in L-lactate, but the mutant strain is still able to grow after a long lag phase. Deletion of both the LldD and LldA genes completely impairs the L-lactate utilization capacity of the strain Pseudomonas aeruginosa
physiological function loss of LldA has little effect on L-lactate utilization. Deletion of both the LldD and LldA genes completely impairs the L-lactate utilization capacity of the strain Pseudomonas aeruginosa