Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-glycerate | strong inhibition | Saccharomyces cerevisiae | |
oxalate | competitive | Saccharomyces cerevisiae | |
oxaloacetate | competitive | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
D-lactate | - |
Saccharomyces cerevisiae | |
1.34 | - |
D-2-hydroxybutyrate | - |
Saccharomyces cerevisiae | |
2.5 | - |
D-2-hydroxyvalerate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
ferricyanide | can substitute for cytochrome c | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | - |
pyruvate + ferrocytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
? | |
(R)-lactate + ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
? | |
D-2-hydroxybutyrate + cytochrome c | - |
Saccharomyces cerevisiae | 2-oxobutanoate + ferrocytochrome c | - |
? | |
D-2-hydroxyvalerate + cytochrome c | very poor substrate | Saccharomyces cerevisiae | 2-oxovalerate + ferrocytochrome c | - |
? | |
DL-glycerate + ferricyanide | 10% of the rate of D-lactate oxidation | Saccharomyces cerevisiae | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
250 | - |
D-lactate | per mol flavin | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
with ferricyanide | Saccharomyces cerevisiae |
7.5 | - |
with cytochrome c | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | low activity with cytochrome c at pH 5.5, almost maximum activity at pH 8.5 | Saccharomyces cerevisiae |
5.5 | 7.5 | very low activity with ferricyanide | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | can substitute for cytochrome c | Saccharomyces cerevisiae | |
cytochrome c | - |
Saccharomyces cerevisiae | |
FAD | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
oxalate | - |
Saccharomyces cerevisiae | |
0.01 | - |
oxaloacetate | - |
Saccharomyces cerevisiae |