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Literature summary for 1.1.2.5 extracted from
- D-Lactate dehydrogenase of Desulfovibrio vulgaris (1981), J. Biochem., 89, 1423-1431.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slight stimulation | Desulfovibrio vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Desulfovibrio vulgaris | |
| cyanide | - |
Desulfovibrio vulgaris | |
| PCMB | - |
Desulfovibrio vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
D-lactate | - |
Desulfovibrio vulgaris | |
| 2.4 | - |
DL-2-hydroxybutyrate | - |
Desulfovibrio vulgaris | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-lactate + ferricytochrome c-553 | Desulfovibrio vulgaris | starting enzyme for carbon and energy metabolism | pyruvate + ferrocytochrome c-553 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Desulfovibrio vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 days, 20% loss of activity | Desulfovibrio vulgaris |
| 4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity | Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-lactate + 1-methoxyphenazinium methyl sulfate | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| D-lactate + 2,6-dichlorophenolindophenol | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| D-lactate + ferricyanide | - |
Desulfovibrio vulgaris | pyruvate + ferrocyanide | - |
? | |
| D-lactate + ferricytochrome c-553 | - |
Desulfovibrio vulgaris | pyruvate + ferrocytochrome c-553 | - |
? | |
| D-lactate + ferricytochrome c-553 | starting enzyme for carbon and energy metabolism | Desulfovibrio vulgaris | pyruvate + ferrocytochrome c-553 | - |
? | |
| D-lactate + methylene blue | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| D-lactate + nitrotetrazolium blue | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| DL-2-hydroxybutyrate + ferricytochrome c-553 | - |
Desulfovibrio vulgaris | 2-ketobutyrate + ferrocytochrome c-553 | - |
? | |
| additional information | no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity | Desulfovibrio vulgaris | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
D-lactate + nitrotetrazolium blue | Desulfovibrio vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue | Desulfovibrio vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferricytochrome c-553 | - |
Desulfovibrio vulgaris | |
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