Literature summary for 1.1.2.7 extracted from

Nojiri, M.; Hira, D.; Yamaguchi, K.; Okajima, T.; Tanizawa, K.; Suzuki, S.
Preparation and characterization of Ca2+-free methanol dehydrogenase from Hyphomicrobium denitrificans A3151 (2005), Chem. Lett., 34, 1036-1037.

No PubMed abstract available

Search for more literature for 1.1.2.7

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis	Hyphomicrobium denitrificans

Protein Variants

Protein Variants	Comment	Organism
additional information	preparation of an inactive Ca2+-free MDH-containing Hyphomicrobium denitrificans strain, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, elucidation of the Ca2+ ion elimination mechanism, overview	Hyphomicrobium denitrificans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	absolutely required, 1.43 Ca2+ per enzyme tetramer, tightly bound to the active site, preparation of Ca2+-free MDH, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, overview, Ca2+-free enzyme is inactive	Hyphomicrobium denitrificans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
9000	-	2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE	Hyphomicrobium denitrificans
65000	-	2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE	Hyphomicrobium denitrificans

Organism

Organism	UniProt	Comment	Textmining
Hyphomicrobium denitrificans	-	-	-
Hyphomicrobium denitrificans A3151	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ca2+-containing and Ca2+-free enzymes from cell-free extracts by ion exchange and hydrophobic interaction chromatography	Hyphomicrobium denitrificans

Reaction

Reaction	Comment	Organism	Reaction ID
a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+	the amino-acid residues related to the active site of MDH, E55, C103, C104, R109, T159, S174, E177, T243, W243, N261, D303, R331, N394, and W476, are completely conserved	Hyphomicrobium denitrificans	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	Ca2+-free enzyme is inactive	Hyphomicrobium denitrificans
13	-	purified reconstituted Ca2+-enzyme	Hyphomicrobium denitrificans
18	-	purified native Ca2+-enzyme	Hyphomicrobium denitrificans

Subunits

Subunits	Comment	Organism
tetramer	2 * 65000, alpha-subunit, + 2 * 9000, beta-subunit, SDS-PAGE	Hyphomicrobium denitrificans

Synonyms

Synonyms	Comment	Organism
MDH	-	Hyphomicrobium denitrificans
methanol dehydrogenase	-	Hyphomicrobium denitrificans

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	bound to the enzyme, preparation of Ca2+-free MDH, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, overview	Hyphomicrobium denitrificans

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Release 2023.1 (January 2023)