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Literature summary for 1.1.2.7 extracted from

  • Grosse, S.; Voigt, C.; Wendlandt, K.D.; Kleber, H.P.
    Purification and properties of methanol dehydrogenase from Methylosinus sp. WI 14 (1998), J. Basic Microbiol., 38, 189-196.
    View publication on PubMed
Search for more literature for 1.1.2.7

Activating Compound

Activating Compound Comment Organism Structure
methylamine can replace NH4+ Methylosinus sp.
NH4+ absolute requirement, NH4+ can be replaced by methylamine but not by di- or triamines Methylosinus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 38% inhibition at 0.1 mM Methylosinus sp.
Fe2+ complete inhibition at 1 mM Methylosinus sp.
additional information no or poor inhibition by Ca2+, Co2+, Li+, Mg2+, Mn2+, and KCN, EDTA, and DTE Methylosinus sp.
phenazine methosulfate 39% inhibition at 3 mM Methylosinus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 sorbic alcohol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
0.45
-
 methanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
0.58
-
 Heptanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
0.65
-
 Octanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
0.79
-
 Hexanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
1.34
-
 Pentanol pH 9.0, 57°C, acceptor 2,6-dichlorophenolindophenol Methylosinus sp.
3.58
-
 ethanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
3.69
-
 Propanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.
6.38
-
 butanol pH 9.0, 57°C, acceptor: 2,6-dichlorophenolindophenol Methylosinus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Methylosinus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 2 * 70000, SDS-PAGE Methylosinus sp.
140000
-
 gel filtration Methylosinus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + ferricytochrome cL Methylosinus sp.
-
 formaldehyde + ferrocytochrome cL
-
 ?

Organism

Organism UniProt Comment Textmining
Methylosinus sp.
-
-
-
Methylosinus sp. WI 14
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 9fold to homogeneity by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration Methylosinus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.12
-
 purified enzyme Methylosinus sp.

Storage Stability

Storage Stability Organism
-18°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 10% of activity after 1 month Methylosinus sp.
4°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 30% of activity after 24 h Methylosinus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. butyraldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
ethanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
heptanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. heptaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
hexanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. hexaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
methanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
methanol + ferricytochrome cL
-
 Methylosinus sp. formaldehyde + ferrocytochrome cL
-
 ?
additional information broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes Methylosinus sp. ?
-
 ?
additional information broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes Methylosinus sp. WI 14 ?
-
 ?
octanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. octaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
pentanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. pentaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
propanol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. propionaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
sorbic alcohol + 2,6-dichlorophenolindophenol with phenazine methosulfate Methylosinus sp. ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Methylosinus sp.

Synonyms

Synonyms Comment Organism
MDH
-
 Methylosinus sp.
methanol dehydrogenase
-
 Methylosinus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
57
-
-
 Methylosinus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min, purified enzyme, loss of 25% activity Methylosinus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Methylosinus sp.

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone prosthetic group, 1.3 molecules per enzyme molecule Methylosinus sp.