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Literature summary for 1.1.2.7 extracted from
- Purification and characterization of a methanol dehydrogenase derived from Methylomicrobium sp. HG-1 cultivated using a compulsory circulation diffusion system (2006), Biotechnol. Bioprocess Eng., 11, 134-139 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM complete inhibition | Methylomicrobium sp. HG-1 |  |
| Cu2+ | 1 mM complete inhibition | Methylomicrobium sp. HG-1 | |
| Fe2+ | 1 mM complete inhibition | Methylomicrobium sp. HG-1 | |
| Mn2+ | 1 mM complete inhibition | Methylomicrobium sp. HG-1 | |
| additional information | 1 mM of Zn2+, Mg2+, and Ca2+ cause complete inhibition, EDTA inhibits in vivo | Methylomicrobium sp. HG-1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
methanol | 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C | Methylomicrobium sp. HG-1 | |
| 5.73 | - |
ethanol | 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C | Methylomicrobium sp. HG-1 | |
| 80.78 | - |
Heptanol | 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C | Methylomicrobium sp. HG-1 | |
| 162 | - |
Hexanol | 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C | Methylomicrobium sp. HG-1 | |
| 172 | - |
Pentanol | 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C | Methylomicrobium sp. HG-1 | |
| 184 | - |
Propanol | 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C | Methylomicrobium sp. HG-1 | |
| 186 | - |
butanol | 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C | Methylomicrobium sp. HG-1 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Methylomicrobium sp. HG-1 | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methanol + 2 oxidized cytochrome cL | Methylomicrobium sp. HG-1 | - |
formaldehyde + 2 reduced cytochrome cL | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylomicrobium sp. HG-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble fraction concentrated with Centricon (Millipore, Billerica, Mass, USA), applied to a POROS 20 HQ column, followed by FPLC Superose 12 HR 10/30 column | Methylomicrobium sp. HG-1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4210 | - |
reduction of 4210 micromol 2,6-dichlorophenol indophenol per min, pH 9.0, 30°C | Methylomicrobium sp. HG-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + 2 reduced cytochrome cL | 7.06% of the activity with methanol | Methylomicrobium sp. HG-1 | ethanol + 2 oxidizeded cytochrome cL | - |
? | |
| butanol + 2 oxidized cytochrome cL | 49.13% of the activity with methanol | Methylomicrobium sp. HG-1 | butanal + 2 reduced cytochrome cL | - |
? | |
| ethanol + 2 oxidized cytochrome cL | 85.13% of the activity with methanol | Methylomicrobium sp. HG-1 | acetaldehyde + 2 reduced cytochrome cL | - |
? | |
| formaldehyde + 2 reduced cytochrome cL | 73.24% of the activity with methanol | Methylomicrobium sp. HG-1 | methanol + 2 oxidizeded cytochrome cL | - |
? | |
| heptanol + 2 oxidized cytochrome cL | 101.88% of the activity with methanol | Methylomicrobium sp. HG-1 | heptanal + 2 reduced cytochrome cL | - |
? | |
| hexanol + 2 oxidized cytochrome cL | 94.10% of the activity with methanol | Methylomicrobium sp. HG-1 | hexanal + 2 reduced cytochrome cL | - |
? | |
| methanol + 2 oxidized cytochrome cL | - |
Methylomicrobium sp. HG-1 | formaldehyde + 2 reduced cytochrome cL | - |
? | |
| octanol + 2 oxidized cytochrome cL | 16.33% of the activity with methanol | Methylomicrobium sp. HG-1 | octanal + 2 reduced cytochrome cL | - |
? | |
| pentanol + 2 oxidized cytochrome cL | 67.40% of the activity with methanol | Methylomicrobium sp. HG-1 | pentanal + 2 reduced cytochrome cL | - |
? | |
| propanol + 2 oxidized cytochrome cL | 59.93% of the activity with methanol | Methylomicrobium sp. HG-1 | propionaldehyde + 2 reduced cytochrome cL | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Methylomicrobium sp. HG-1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Methylomicrobium sp. HG-1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome cL | - |
Methylomicrobium sp. HG-1 |
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