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Literature summary for 1.1.2.7 extracted from

  • Kim, H.G.; Kim, S.W.
    Purification and characterization of a methanol dehydrogenase derived from Methylomicrobium sp. HG-1 cultivated using a compulsory circulation diffusion system (2006), Biotechnol. Bioprocess Eng., 11, 134-139 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
Cu2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
Fe2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
Mn2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
additional information 1 mM of Zn2+, Mg2+, and Ca2+ cause complete inhibition, EDTA inhibits in vivo Methylomicrobium sp. HG-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.81
-
methanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
5.73
-
ethanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
80.78
-
Heptanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
162
-
Hexanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
172
-
Pentanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
184
-
Propanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
186
-
butanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Methylomicrobium sp. HG-1 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + 2 oxidized cytochrome cL Methylomicrobium sp. HG-1
-
formaldehyde + 2 reduced cytochrome cL
-
?

Organism

Organism UniProt Comment Textmining
Methylomicrobium sp. HG-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble fraction concentrated with Centricon (Millipore, Billerica, Mass, USA), applied to a POROS 20 HQ column, followed by FPLC Superose 12 HR 10/30 column Methylomicrobium sp. HG-1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4210
-
reduction of 4210 micromol 2,6-dichlorophenol indophenol per min, pH 9.0, 30°C Methylomicrobium sp. HG-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + 2 reduced cytochrome cL 7.06% of the activity with methanol Methylomicrobium sp. HG-1 ethanol + 2 oxidizeded cytochrome cL
-
?
butanol + 2 oxidized cytochrome cL 49.13% of the activity with methanol Methylomicrobium sp. HG-1 butanal + 2 reduced cytochrome cL
-
?
ethanol + 2 oxidized cytochrome cL 85.13% of the activity with methanol Methylomicrobium sp. HG-1 acetaldehyde + 2 reduced cytochrome cL
-
?
formaldehyde + 2 reduced cytochrome cL 73.24% of the activity with methanol Methylomicrobium sp. HG-1 methanol + 2 oxidizeded cytochrome cL
-
?
heptanol + 2 oxidized cytochrome cL 101.88% of the activity with methanol Methylomicrobium sp. HG-1 heptanal + 2 reduced cytochrome cL
-
?
hexanol + 2 oxidized cytochrome cL 94.10% of the activity with methanol Methylomicrobium sp. HG-1 hexanal + 2 reduced cytochrome cL
-
?
methanol + 2 oxidized cytochrome cL
-
Methylomicrobium sp. HG-1 formaldehyde + 2 reduced cytochrome cL
-
?
octanol + 2 oxidized cytochrome cL 16.33% of the activity with methanol Methylomicrobium sp. HG-1 octanal + 2 reduced cytochrome cL
-
?
pentanol + 2 oxidized cytochrome cL 67.40% of the activity with methanol Methylomicrobium sp. HG-1 pentanal + 2 reduced cytochrome cL
-
?
propanol + 2 oxidized cytochrome cL 59.93% of the activity with methanol Methylomicrobium sp. HG-1 propionaldehyde + 2 reduced cytochrome cL
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Methylomicrobium sp. HG-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Methylomicrobium sp. HG-1

Cofactor

Cofactor Comment Organism Structure
cytochrome cL
-
Methylomicrobium sp. HG-1