BRENDA - Enzyme Database
show all sequences of 1.1.2.B2

Factors affecting the stability of methanol dehydrogenase from Paracoccus denitrificans

Davidson, V.L.; Wu, J.; Miller, B.; Jones, L.H.; FEMS Microbiol. Lett. 94, 53-58 (1992)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
decreases enzyme thermal stability
Paracoccus denitrificans
KCN
-
Paracoccus denitrificans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Paracoccus denitrificans
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
increases enzyme thermal stability, Ca2+ has a structural role
Paracoccus denitrificans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
x * 67000 + x * 12000, SDS-PAGE
Paracoccus denitrificans
67000
-
x * 67000 + x * 12000, SDS-PAGE
Paracoccus denitrificans
Organism
Organism
UniProt
Commentary
Textmining
Paracoccus denitrificans
-
strain ATCC 13543
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme 6.8fold from periplasm to homogeneity by ultrafiltration, dialysis, anion exchange chromatography, and gel filtration
Paracoccus denitrificans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:methanol-grown cell
-
Paracoccus denitrificans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4
-
purified enzyme
Paracoccus denitrificans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
methanol + nitroblue tetrazolium
-
686814
Paracoccus denitrificans
formaldehyde + reduced nitroblue tetrazolium
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 67000 + x * 12000, SDS-PAGE
Paracoccus denitrificans
Synonyms
Synonyms
Commentary
Organism
methanol dehydrogenase
-
Paracoccus denitrificans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Paracoccus denitrificans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
10
60
10 min, the purified enzyme is quite stable within this range, significant loss of activity occurs at higher temperatures, Ca2+ enhances the thermal stability, while EDTA reduces it
Paracoccus denitrificans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
and above
Paracoccus denitrificans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
11.5
-
Paracoccus denitrificans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4.5
9
30°C, 20 h, stable below pH 9, rapid loss of activity above pH 9
Paracoccus denitrificans
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
non-covalently bound
Paracoccus denitrificans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
non-covalently bound
Paracoccus denitrificans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
decreases enzyme thermal stability
Paracoccus denitrificans
KCN
-
Paracoccus denitrificans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Paracoccus denitrificans
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
increases enzyme thermal stability, Ca2+ has a structural role
Paracoccus denitrificans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
x * 67000 + x * 12000, SDS-PAGE
Paracoccus denitrificans
67000
-
x * 67000 + x * 12000, SDS-PAGE
Paracoccus denitrificans
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 6.8fold from periplasm to homogeneity by ultrafiltration, dialysis, anion exchange chromatography, and gel filtration
Paracoccus denitrificans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:methanol-grown cell
-
Paracoccus denitrificans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4
-
purified enzyme
Paracoccus denitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
methanol + nitroblue tetrazolium
-
686814
Paracoccus denitrificans
formaldehyde + reduced nitroblue tetrazolium
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 67000 + x * 12000, SDS-PAGE
Paracoccus denitrificans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Paracoccus denitrificans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
10
60
10 min, the purified enzyme is quite stable within this range, significant loss of activity occurs at higher temperatures, Ca2+ enhances the thermal stability, while EDTA reduces it
Paracoccus denitrificans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
and above
Paracoccus denitrificans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
11.5
-
Paracoccus denitrificans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4.5
9
30°C, 20 h, stable below pH 9, rapid loss of activity above pH 9
Paracoccus denitrificans
Other publictions for EC 1.1.2.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
687828
Xia
X-ray structure of methanol de ...
Paracoccus denitrificans
J. Biol. Inorg. Chem.
8
843-854
2003
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1
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1
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1
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1
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2
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3
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-
-
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-
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1
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-
-
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1
1
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-
1
-
1
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-
-
-
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-
-
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2
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-
-
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-
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-
685051
Harris
A new kinetic model for the st ...
Paracoccus denitrificans
Biochemistry
32
4362-4368
1993
2
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3
6
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1
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1
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1
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7
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1
1
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1
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1
2
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2
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1
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3
2
6
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1
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1
-
7
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1
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-
-
1
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-
-
-
-
-
-
-
-
686814
Davidson
Factors affecting the stabilit ...
Paracoccus denitrificans
FEMS Microbiol. Lett.
94
53-58
1992
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2
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1
1
2
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1
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1
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1
1
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1
1
1
1
-
1
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1
1
1
1
-
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1
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2
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1
1
2
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1
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1
1
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1
1
1
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1
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1
1
1
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