BRENDA - Enzyme Database
show all sequences of 1.1.2.B2

X-ray structure of methanol dehydrogenase from Paracoccus denitrificans and molecular modeling of its interactions with cytochrome c-551i

Xia, Z.X.; Dai, W.W.; He, Y.N.; White, S.A.; Mathews, F.S.; Davidson, V.L.; J. Biol. Inorg. Chem. 8, 843-854 (2003)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
purified enzyme, hanging drop vapor diffusion and macro-seeding, mixing of protein solution containing 5 mg/ml protein, 0.1 M Tris-HCl, pH 8.3, 0.2 M Li2SO4, 30% PEG 3350, with precipitant solution containing 0.1 M Tris-HCl, pH 8.5, 30% PEG 4000, 0.2 M Li2SO4, several days, X-ray diffraction structure determination and analysis at 2.5 A resolution, modelling
Paracoccus denitrificans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Paracoccus denitrificans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
methanol + ferricytochrome c551i
Paracoccus denitrificans
the primary electron acceptor of MEDH-PD is cytochrome c-551i
formaldehyde + ferrocytochrome c551i
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Paracoccus denitrificans
P12293
P12293 (large subunit, alpha) and P12293 (small subunit, beta)
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
methanol + ferricytochrome c551i
the primary electron acceptor of MEDH-PD is cytochrome c-551i
687828
Paracoccus denitrificans
formaldehyde + ferrocytochrome c551i
-
-
-
?
additional information
interaction of the enzyme with cytochrome c551i, and electron transfer from reduced pyrroloquinoline quinone to the cytochrome, interface structure, modelling, overview
687828
Paracoccus denitrificans
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
MEDH
-
Paracoccus denitrificans
MEDH-PD
-
Paracoccus denitrificans
methanol dehydrogenase
-
Paracoccus denitrificans
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Paracoccus denitrificans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Paracoccus denitrificans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified enzyme, hanging drop vapor diffusion and macro-seeding, mixing of protein solution containing 5 mg/ml protein, 0.1 M Tris-HCl, pH 8.3, 0.2 M Li2SO4, 30% PEG 3350, with precipitant solution containing 0.1 M Tris-HCl, pH 8.5, 30% PEG 4000, 0.2 M Li2SO4, several days, X-ray diffraction structure determination and analysis at 2.5 A resolution, modelling
Paracoccus denitrificans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Paracoccus denitrificans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
methanol + ferricytochrome c551i
Paracoccus denitrificans
the primary electron acceptor of MEDH-PD is cytochrome c-551i
formaldehyde + ferrocytochrome c551i
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
methanol + ferricytochrome c551i
the primary electron acceptor of MEDH-PD is cytochrome c-551i
687828
Paracoccus denitrificans
formaldehyde + ferrocytochrome c551i
-
-
-
?
additional information
interaction of the enzyme with cytochrome c551i, and electron transfer from reduced pyrroloquinoline quinone to the cytochrome, interface structure, modelling, overview
687828
Paracoccus denitrificans
?
-
-
-
?
Other publictions for EC 1.1.2.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
687828
Xia
X-ray structure of methanol de ...
Paracoccus denitrificans
J. Biol. Inorg. Chem.
8
843-854
2003
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1
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1
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1
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1
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2
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3
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1
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1
1
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1
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1
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2
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685051
Harris
A new kinetic model for the st ...
Paracoccus denitrificans
Biochemistry
32
4362-4368
1993
2
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3
6
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1
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1
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1
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7
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1
1
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1
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1
2
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2
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1
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3
2
6
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1
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1
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7
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1
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1
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686814
Davidson
Factors affecting the stabilit ...
Paracoccus denitrificans
FEMS Microbiol. Lett.
94
53-58
1992
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2
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1
1
2
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1
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1
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1
1
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1
1
1
1
-
1
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1
1
1
1
-
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1
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2
-
-
1
1
2
-
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1
-
1
1
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1
1
1
-
1
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1
1
1
-
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