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Literature summary for 1.1.3.10 extracted from

  • Dietrich, D.; Crooks, C.
    Gene cloning and heterologous expression of pyranose 2-oxidase from the brown-rot fungus, Gloeophyllum trabeum (2009), Biotechnol. Lett., 31, 1223-1228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed heterologously in Escherichia coli DH5alpha and BL21(DE3) Gloeophyllum trabeum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
predicted from amino acid sequence, open reading frame of 1962 base pairs encoding a 653 amino acid polypeptide Gloeophyllum trabeum

Organism

Organism UniProt Comment Textmining
Gloeophyllum trabeum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
heterologously expressed enzyme yields 15 U enzyme activity per ml culture, 15 U substrate glucose, 2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) assay, pH 6.5, 25°C Gloeophyllum trabeum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + O2
-
Gloeophyllum trabeum 2-dehydro-D-glucose + H2O2
-
?

Synonyms

Synonyms Comment Organism
pyranose 2-Oxidase
-
Gloeophyllum trabeum