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Literature summary for 1.1.3.10 extracted from

  • Tan, T.C.; Pitsawong, W.; Wongnate, T.; Spadiut, O.; Haltrich, D.; Chaiyen, P.; Divne, C.
    H-bonding and positive charge at the N5/O4 locus are critical for covalent flavin attachment in trametes pyranose 2-oxidase (2010), J. Mol. Biol., 402, 578-594.
    View publication on PubMed
Search for more literature for 1.1.3.10

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in Escherichia coli XL1-blue cells Trametes ochracea

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes H167A and T169, Ahanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 4.6), 50 mM MgCl2, and 6% (w/v) monomethyl ether PEG 2000 Trametes ochracea

Protein Variants

Protein Variants Comment Organism
H167A FAD-deficient mutant Trametes ochracea
H548A partially FAD-deficient mutant Trametes ochracea
H548N partially FAD-deficient mutant Trametes ochracea
T169A FAD-deficient mutant Trametes ochracea
T169G the FAD content of the mutant enzyme is similar to the wild type enzyme Trametes ochracea
T169N the FAD content of the mutant enzyme is similar to the wild type enzyme Trametes ochracea
T169S the FAD content of the mutant enzyme is similar to the wild type enzyme Trametes ochracea

Inhibitors

Inhibitors Comment Organism Structure
acetate competitive inhibitor Trametes ochracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Trametes ochracea
-
 2-dehydro-D-glucose + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Trametes ochracea Q7ZA32 synonym Trametes ochracea
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + O2
-
 Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?

Subunits

Subunits Comment Organism
homotetramer X-ray crystallograhy Trametes ochracea

Synonyms

Synonyms Comment Organism
P2O
-
 Trametes ochracea
pyranose 2-Oxidase
-
 Trametes ochracea

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Trametes ochracea