Any feedback?
Literature summary for 1.1.3.10 extracted from
- Structural basis for binding of fluorinated glucose and galactose to Trametes multicolor pyranose 2-oxidase variants with improved galactose conversion (2014), PLoS ONE, 9, e86736.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 0.1 M MES (pH 5.2), 50 mM MgCl2, and 10% (w/v) monomethylether polyethylene glycol 2000 | Trametes ochracea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H167A | the mutant exhibits reduced D-glucose conversion and no D-galactose conversion activity | Trametes ochracea |
| H450G | the mutant exhibits reduced D-glucose conversion and improved D-galactose conversion activity | Trametes ochracea |
| H450G/V546C | the mutant exhibits reduced D-glucose conversion and improved D-galactose conversion activity | Trametes ochracea |
| T169G | the mutant exhibits reduced D-glucose conversion and D-galactose conversion activities | Trametes ochracea |
| T196G/V546C | the mutant exhibits reduced D-glucose conversion and D-galactose conversion activities | Trametes ochracea |
| V546C | the mutant exhibits reduced D-glucose and D-galactose conversion activities | Trametes ochracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
D-galactose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea |  |
| 0.44 | - |
D-glucose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.69 | - |
D-glucose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.939 | - |
D-glucose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.987 | - |
D-glucose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
| 2.43 | - |
D-glucose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 2.45 | - |
D-galactose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
| 2.48 | - |
D-galactose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
| 3.06 | - |
D-glucose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 5.97 | - |
D-glucose | mutant enzyme H167A, pH and temperature not specified in the publication | Trametes ochracea | |
| 8.79 | - |
D-galactose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
| 12 | - |
D-galactose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 46.2 | - |
D-galactose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + O2 | Trametes ochracea | - |
2-dehydro-D-glucose + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes ochracea | Q7ZA32 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography | Trametes ochracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + O2 | - |
Trametes ochracea | 2-dehydro-D-galactose + H2O2 | - |
? | |
| D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P2O | - |
Trametes ochracea |
| pyranose 2-Oxidase | - |
Trametes ochracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
D-glucose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.27 | - |
D-galactose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.38 | - |
D-galactose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.43 | - |
D-glucose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 2.06 | - |
D-glucose | mutant enzyme H167A, pH and temperature not specified in the publication | Trametes ochracea | |
| 2.51 | - |
D-galactose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
| 3.51 | - |
D-galactose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
| 5.92 | - |
D-galactose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 6.57 | - |
D-galactose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 12.5 | - |
D-glucose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
| 16.8 | - |
D-glucose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 48.1 | - |
D-glucose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
| 88.6 | - |
D-glucose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
D-galactose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.142 | - |
D-galactose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.286 | - |
D-galactose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.35 | - |
D-glucose | mutant enzyme H167A, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.38 | - |
D-glucose | mutant enzyme T169G, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.493 | - |
D-galactose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.94 | - |
D-galactose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 0.99 | - |
D-glucose | mutant enzyme T196G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 1.43 | - |
D-galactose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
| 12.7 | - |
D-glucose | mutant enzyme H450G, pH and temperature not specified in the publication | Trametes ochracea | |
| 13.5 | - |
D-glucose | mutant enzyme H450G/V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 29 | - |
D-glucose | mutant enzyme V546C, pH and temperature not specified in the publication | Trametes ochracea | |
| 51.2 | - |
D-glucose | wild type enzyme, pH and temperature not specified in the publication | Trametes ochracea | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
