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Literature summary for 1.1.3.13 extracted from

  • Bringer, S.; Sprey, B.; Sahm, H.
    Purification and properties of alcohol oxidase from Poria contigua (1979), Eur. J. Biochem., 101, 563-570.
    View publication on PubMed
Search for more literature for 1.1.3.13

General Stability

General Stability Organism
freezing at -20°C and thawing leads to 25% loss of activity Poria contigua

Inhibitors

Inhibitors Comment Organism Structure
NaN3 strong Poria contigua
PCMB 1 mM, complete inhibition Poria contigua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 methanol
-
 Poria contigua
1
-
 ethanol
-
 Poria contigua
3.2
-
 2-propin-1-ol
-
 Poria contigua
6.1
-
 formaldehyde
-
 Poria contigua
8.3
-
 n-Propanol
-
 Poria contigua
21.3
-
 n-butanol
-
 Poria contigua
25
-
 Isopropanol
-
 Poria contigua
27.2
-
 2-mercaptoethanol
-
 Poria contigua

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Poria contigua 5737
-
microbody
-
 Poria contigua 42579
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no iron cofactor Poria contigua

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
 8 * 79000, SDS-PAGE Poria contigua
610000
-
 equilibrium sedimentation Poria contigua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 Poria contigua key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Poria contigua
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Poria contigua

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Poria contigua
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.7
-
-
 Poria contigua

Storage Stability

Storage Stability Organism
4°C, purified, stable for 2 weeks Poria contigua
4°C, storage in presence of antimicrobial agent sodium azide, 0.01% in 50 mM potassium phosphate buffer, pH 7.7, stable for at least 2 weeks Poria contigua

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-mercaptoethanol + O2
-
 Poria contigua 2-mercaptoethanal + H2O2
-
 ?
2-propyn-1-ol + O2
-
 Poria contigua propyn-1-al + H2O2
-
 ?
2-propyn-1-ol + O2
-
 Poria contigua 2-propyn-1-al + H2O2
-
 ?
ethanol + O2
-
 Poria contigua acetaldehyde + H2O2
-
 ?
formaldehyde + O2
-
 Poria contigua formate + H2O2
-
 ?
isopropanol + O2
-
 Poria contigua 2-propanone + H2O2
-
 ?
methanol + O2
-
 Poria contigua formaldehyde + H2O2
-
 ?
methanol + O2 key enzyme of methanol metabolism Poria contigua formaldehyde + H2O2
-
 ?
n-butanol + O2
-
 Poria contigua n-butanal + H2O2
-
 ?
n-propanol + O2
-
 Poria contigua n-propanal + H2O2
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 79000, SDS-PAGE Poria contigua

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol FAD per mol of subunit Poria contigua

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
 Sodium azide
-
 Poria contigua