General Stability | Organism |
---|---|
7.7 mM NaN3, 30°C, enzyme retains activity for at least 35 days, inactivated completely after 10 days without NaN3 | [Candida] methanosorbosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM CuSO4, 30 min, strong | [Candida] methanosorbosa | |
Hg2+ | 1 mM HgCl2, 30 min, strong | [Candida] methanosorbosa | |
hydroquinone | 1 mM, 30 min, strong | [Candida] methanosorbosa | |
NEM | 1 mM, 30 min, strong | [Candida] methanosorbosa | |
PCMB | 1 mM, 30 min, strong | [Candida] methanosorbosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.43 | - |
methanol | - |
[Candida] methanosorbosa | |
8.23 | - |
ethanol | - |
[Candida] methanosorbosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
8 * 80000, SDS-PAGE | [Candida] methanosorbosa |
620000 | - |
gel filtration | [Candida] methanosorbosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] methanosorbosa | - |
M-2003 | - |
[Candida] methanosorbosa M-2003 | - |
M-2003 | - |
Purification (Comment) | Organism |
---|---|
- |
[Candida] methanosorbosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
[Candida] methanosorbosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.11 | - |
- |
[Candida] methanosorbosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-octanol + O2 | 2.4% of the activity with methanol | [Candida] methanosorbosa | n-octanal + H2O2 | - |
? | |
1-pentanol + O2 | 33.5% of the activity with methanol | [Candida] methanosorbosa | n-pentanal + H2O2 | - |
? | |
1-pentanol + O2 | 33.5% of the activity with methanol | [Candida] methanosorbosa M-2003 | n-pentanal + H2O2 | - |
? | |
2-butanol + O2 | 3.5% of the activity with methanol | [Candida] methanosorbosa | 2-butanone + H2O2 | - |
? | |
2-butanol + O2 | 3.5% of the activity with methanol | [Candida] methanosorbosa M-2003 | 2-butanone + H2O2 | - |
? | |
benzyl alcohol + O2 | 21.3% of the activity with methanol | [Candida] methanosorbosa | benzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | 21.3% of the activity with methanol | [Candida] methanosorbosa M-2003 | benzaldehyde + H2O2 | - |
? | |
ethanol + O2 | 97.8% of the activity with methanol | [Candida] methanosorbosa | acetaldehyde + H2O2 | - |
? | |
ethanol + O2 | 97.8% of the activity with methanol | [Candida] methanosorbosa M-2003 | acetaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
[Candida] methanosorbosa | formaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
[Candida] methanosorbosa M-2003 | formaldehyde + H2O2 | - |
? | |
n-butanol + O2 | 52.2% of the activity with methanol | [Candida] methanosorbosa | n-butanal + H2O2 | - |
? | |
n-hexanol + O2 | 6.3% of the activity with methanol | [Candida] methanosorbosa | n-hexanal + H2O2 | - |
? | |
n-propanol + O2 | 77.5% of the activity with methanol | [Candida] methanosorbosa | n-propanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 80000, SDS-PAGE | [Candida] methanosorbosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
[Candida] methanosorbosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 6.0-8.5. 60 min, stable | [Candida] methanosorbosa |
50 | - |
pH 7.5, 15 min, stable below | [Candida] methanosorbosa |
55 | - |
15 min, 34% loss of activity | [Candida] methanosorbosa |
65 | - |
15 min, complete inactivation above | [Candida] methanosorbosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
[Candida] methanosorbosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
[Candida] methanosorbosa |