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Literature summary for 1.1.3.13 extracted from

  • Kumar, A.K.; Goswami, P.
    Functional characterization of alcohol oxidases from Aspergillus terreus MTCC 6324 (2006), Appl. Microbiol. Biotechnol., 72, 906-911.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
n-Heptanol pH 8.5, 30°C, SCAO Aspergillus terreus
0.028
-
n-butanol pH 8.5, 30°C, SCAO Aspergillus terreus
0.05
-
n-Octanol pH 8.5, 30°C, SCAO Aspergillus terreus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Aspergillus terreus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
272000
-
above, native PAGE Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + O2 Aspergillus terreus very low activity in cells grown on n-hexadecane acetaldehyde + H2O2
-
?
methanol + O2 Aspergillus terreus very low activity in cells grown on n-hexadecane formaldehyde + H2O2
-
?
n-butanol + O2 Aspergillus terreus
-
butanaldehyde + H2O2
-
?
n-heptanol + O2 Aspergillus terreus
-
heptanaldehyde + H2O2
-
?
n-octanol + O2 Aspergillus terreus
-
octanaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-
Aspergillus terreus MTCC 6324
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity Aspergillus terreus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:n-hexadecane-grown cell
-
Aspergillus terreus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Aspergillus terreus

Storage Stability

Storage Stability Organism
4°C, purified enzyme, 24 h, loss of only 5% of activity Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + O2
-
Aspergillus terreus acetaldehyde + H2O2
-
?
ethanol + O2 very low activity in cells grown on n-hexadecane Aspergillus terreus acetaldehyde + H2O2
-
?
methanol + O2
-
Aspergillus terreus formaldehyde + H2O2
-
?
methanol + O2 very low activity in cells grown on n-hexadecane Aspergillus terreus formaldehyde + H2O2
-
?
methanol + O2
-
Aspergillus terreus MTCC 6324 formaldehyde + H2O2
-
?
additional information substrate specificity dependent on growth substrate, overview Aspergillus terreus ?
-
?
additional information substrate specificity dependent on growth substrate, overview Aspergillus terreus MTCC 6324 ?
-
?
n-butanol + O2
-
Aspergillus terreus butanaldehyde + H2O2
-
?
n-heptanol + O2
-
Aspergillus terreus heptanaldehyde + H2O2
-
?
n-octanol + O2
-
Aspergillus terreus octanaldehyde + H2O2
-
?

Synonyms

Synonyms Comment Organism
ethanol oxidase
-
Aspergillus terreus
methanol oxidase
-
Aspergillus terreus
SCAO
-
Aspergillus terreus
short chain alcohol oxidase
-
Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
24 h, loss of only 5% of activity Aspergillus terreus
30
-
the enzyme is highly temperature-sensitive, loss of 20% activity within 100 min Aspergillus terreus
35
-
30 min, loss of 50% activity, inactivation after 100 min Aspergillus terreus
40
-
inactivation after 30 min Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Aspergillus terreus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable at pH 9.0, loss of 60% activity at pH 6.0, the activity is drastically reduced at below neutral pH and above pH 9.0, overview Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
FAD bound Aspergillus terreus