Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydrazine | 1 mM, complete inhibition | Aspergillus ochraceus | |
hydroxylamine | 1 mM, complete inhibition | Aspergillus ochraceus | |
Monoiodoacetic acid | 1 mM, 31% inhibition | Aspergillus ochraceus | |
phenylhydrazine | 1 mM, complete inhibition | Aspergillus ochraceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
ethylene glycol | - |
Aspergillus ochraceus | |
12 | - |
ethanol | - |
Aspergillus ochraceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
4 * 68000, SDS-PAGE | Aspergillus ochraceus |
262000 | - |
gel filtration | Aspergillus ochraceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus ochraceus | - |
AIU 031 | - |
Aspergillus ochraceus AIU 031 | - |
AIU 031 | - |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus ochraceus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition | when incubated in the cholesterol medium, the alcohol oxidase activity reaches the maximum at 2 days of cultivation, and then gradually decreases. Methanol, ethanol and isopropanol are also effective for production of alcohol oxidase, but glycerol, glucose and malic acid are not | Aspergillus ochraceus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.59 | - |
oxidation of ethanol | Aspergillus ochraceus |
3.64 | - |
oxidation of ethylene glycol | Aspergillus ochraceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + O2 | 49% of the activity with methanol | Aspergillus ochraceus | n-butanal + H2O2 | - |
? | |
1-butanol + O2 | 49% of the activity with methanol | Aspergillus ochraceus AIU 031 | n-butanal + H2O2 | - |
? | |
1-propanol + O2 | 66% of the activity with methanol | Aspergillus ochraceus | n-propanal + H2O2 | - |
? | |
1-propanol + O2 | 66% of the activity with methanol | Aspergillus ochraceus AIU 031 | n-propanal + H2O2 | - |
? | |
ethanol + O2 | 97% of the activity with methanol | Aspergillus ochraceus | acetaldehyde + H2O2 | - |
? | |
ethanol + O2 | 97% of the activity with methanol | Aspergillus ochraceus AIU 031 | acetaldehyde + H2O2 | - |
? | |
ethylene glycol + O2 | 12% of the activity with methanol | Aspergillus ochraceus | ? | - |
? | |
ethylene glycol + O2 | 12% of the activity with methanol | Aspergillus ochraceus AIU 031 | ? | - |
? | |
methanol + O2 | - |
Aspergillus ochraceus | formaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
Aspergillus ochraceus AIU 031 | formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 68000, SDS-PAGE | Aspergillus ochraceus |
Synonyms | Comment | Organism |
---|---|---|
AOd | - |
Aspergillus ochraceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | ethanol oxidation | Aspergillus ochraceus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
0.1 M potassium phosphate buffer, pH 7.0, 20 min, almost all the enzyme activity remains | Aspergillus ochraceus |
50 | - |
0.1 M potassium phosphate buffer, pH 7.0, 20 min, about 60% of the activity remains | Aspergillus ochraceus |
55 | - |
0.1 M potassium phosphate buffer, pH 7.0, 20 min, about 25% of the activity remains | Aspergillus ochraceus |
60 | - |
0.1 M potassium phosphate buffer, pH 7.0, 20 min, complete inactivation | Aspergillus ochraceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | ethanol oxidation | Aspergillus ochraceus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
40°C, 20 min, about 60% loss of activity | Aspergillus ochraceus |
6.5 | 8.5 | 40°C, 20 min, more than 90% of the activity remains | Aspergillus ochraceus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus ochraceus | isoelectric focusing, pH-range: 3.5-10 | - |
5.4 |