Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | 17.5-26% activation at 1 mM | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | strong inhibition at 0.01 mM | Rattus sp. | |
8-hydroxyquinoline | 8-17% inhibition at 1 mM | Rattus sp. | |
Cu2+ | complete inhibition at 0.025 mM | Rattus sp. | |
diethyldithiocarbamate | 51-55% inhibition at 1 mM | Rattus sp. | |
KCN | 30% inhibition of L-2-hydroxyisocaproate oxidation at 1 mM, 91% inhibition of glycolate oxidation at 1 mM | Rattus sp. | |
o-Iodosobenzoate | 46% inhibition at 0.1 mM | Rattus sp. | |
o-phenanthroline | 5-14% inhibition at 1 mM | Rattus sp. | |
Quinacrine | 6% inhibition at 1 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
glycolate | - |
Rattus sp. | |
1.34 | - |
DL-2-hydroxycaproate | - |
Rattus sp. | |
1.65 | - |
L-2-Hydroxyisocaproate | - |
Rattus sp. | |
8.5 | - |
L-lactate | - |
Rattus sp. | |
12.7 | - |
DL-2-hydroxybutyrate | - |
Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lactate + O2 | Rattus sp. | traces of activity | pyruvate + H2O2 | - |
? | |
DL-2-hydroxybutyrate + O2 | Rattus sp. | - |
2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | Rattus sp. | - |
2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + O2 | Rattus sp. | low activity | 2-oxoisovalerate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | Rattus sp. | - |
2-oxovalerate + H2O2 | - |
? | |
glycolate + O2 | Rattus sp. | - |
glyoxylate + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | Rattus sp. | best substrate tested | 2-oxoisocaproate + H2O2 | - |
? | |
L-lactate + O2 | Rattus sp. | - |
pyruvate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.43 | - |
- |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lactate + O2 | traces of activity | Rattus sp. | pyruvate + H2O2 | - |
? | |
DL-2-hydroxybutyrate + O2 | - |
Rattus sp. | 2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | - |
Rattus sp. | 2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + O2 | low activity | Rattus sp. | 2-oxoisovalerate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | - |
Rattus sp. | 2-oxovalerate + H2O2 | - |
? | |
glycolate + O2 | - |
Rattus sp. | glyoxylate + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | best substrate tested | Rattus sp. | 2-oxoisocaproate + H2O2 | - |
? | |
L-lactate + O2 | - |
Rattus sp. | pyruvate + H2O2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
diethyldithiocarbamate | competitive | Rattus sp. | |
0.88 | - |
KCN | uncompetitive | Rattus sp. |