KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Zea mays | |
additional information | - |
additional information | Michaelis-Menten kinetics | Glycine max | |
0.02 | - |
glycolate | mesophyll cell enzyme | Zea mays | |
0.056 | - |
glycolate | bundle sheath cell enzyme | Zea mays | |
0.06 | - |
glycolate | leaf enzyme | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 x 37000 + 2 * 44000, SDS-PAGE | Zea mays |
160000 | - |
sequence calculation | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + O2 | Zea mays | - |
glyoxylate + H2O2 | - |
? | |
glycolate + O2 | Glycine max | - |
glyoxylate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
cv. Pripyat, various isozymes | - |
Zea mays | - |
cv. Voronezhskaya 76, various isozymes | - |
Purification (Comment) | Organism |
---|---|
native enzyme from leaves 207fold by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration | Glycine max |
native enzyme from maize leaf bundle sheath 46.6fold by gel filtration, anion exchange chromatography, and again gel filtration. Native enzyme 10.5fold from leaf mesophyll, by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bundle sheath | - |
Zea mays | - |
leaf | - |
Zea mays | - |
leaf | - |
Glycine max | - |
mesophyll | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.65 | - |
purified native enzyme from leaves | Zea mays |
1546 | - |
purified native enzyme from mesophyll bundle sheath | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + O2 | - |
Zea mays | glyoxylate + H2O2 | - |
? | |
glycolate + O2 | - |
Glycine max | glyoxylate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | quaternary structure of C3 plant GO, overview | Zea mays |
octamer | - |
Glycine max |
tetramer | 2 x 37000 + 2 * 44000, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
glycolate oxidase | - |
Zea mays |
glycolate oxidase | - |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the dependence of GO activity on pH is bell-shaped | Zea mays |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the metabolism of glycolate | Glycine max |
metabolism | the enzyme is involved in the metabolism of glycolate, glycolate oxidase is a key enzyme involved in C3 photorespiration metabolic pathway, the process where plants lose up to half of assimilated carbon | Zea mays |
physiological function | role in C3 and C4 plants and associated regulation mechanisms | Zea mays |
physiological function | role in C3 and C4 plants and associated regulation mechanisms | Glycine max |