Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + O2 | Bombyx mori | inactivation of ecdysone | 3-dehydroecdysone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | H6AGY0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut | also in midgut goblet cell cavities | Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + O2 | - |
Bombyx mori | 3-dehydroecdysone + H2O2 | - |
? | |
ecdysone + O2 | inactivation of ecdysone | Bombyx mori | 3-dehydroecdysone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | Bombyx mori |
General Information | Comment | Organism |
---|---|---|
metabolism | ecdysone is inactivated to 3-dehydroecdysone under ecdysone oxidase, followed by NAD(P)H-dependent irreversible reduction to 3-epiecdysteroid under 3-dehydroecdysone 3alpha-reductase. On the other hand, 3-dehydroecdysone undergoes reversible reduction to ecdysone by 3-dehydroecdysone 3beta-reductase in the hemolymph | Bombyx mori |