Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3-dimethylbutyraldehyde | product analogue inhibition, 74-90% reduced flavin reduction dependent on pH | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant enzyme, NMR-determination of hydration ratio of betaine aldehyde, overview | Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | Arthrobacter globiformis | the enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde in two sequential FAD-dependent reaction steps, overview | betaine aldehyde + H2O2 | - |
? | |
FADH2 + O2 | Arthrobacter globiformis | - |
FAD + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
strain ATCC 8010 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
betaine aldehyde + O2 + H2O = betaine + H2O2 | reaction mechanism, the enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde in two sequential FAD-dependent reaction steps | Arthrobacter globiformis | |
choline + O2 = betaine aldehyde + H2O2 | reaction mechanism, the enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde in two sequential FAD-dependent reaction steps | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3-dimethylbutan-1-ol + O2 | 10fold lower activity compared to choline | Arthrobacter globiformis | ? | - |
? | |
choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? | |
choline + O2 | the enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde in two sequential FAD-dependent reaction steps, overview | Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? | |
FADH2 + O2 | - |
Arthrobacter globiformis | FAD + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHO | - |
Arthrobacter globiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arthrobacter globiformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound to the enzyme, complete reduction of the enzyme-bound flavin is observed in a stopped-flow spectrophotometer upon anaerobic mixing with betaine aldehyde or choline at pH 8.0, with similar kred values | Arthrobacter globiformis |