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Literature summary for 1.1.3.17 extracted from
- An internal equilibrium preorganizes the enzyme-substrate complex for hydride tunneling in choline oxidase (2007), Biochemistry, 46, 6402-6408.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli from plasmid pET/codA1 | Arthrobacter globiformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + O2 | Arthrobacter globiformis | - |
betaine aldehyde + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter globiformis | Q7X2H8 | strain ATCC 8010 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Arthrobacter globiformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
comparison of thermodynamic and kinetic parameters of the hydride transfer reaction under reversible and irreversible catalytic regimes, significantly larger enthalpies of activation in the reversible catalytic regime, first example of the use of temperature-dependent kinetic isotope effects to measure thermodynamic parameters for hydride ion tunneling associated with the presence of conformational changes occurring in the enzyme-substrate complex | Arthrobacter globiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? | |
| choline + O2 | analysis of asynchronous hydride transfer mechanism for choline oxidation | Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 45 | monitoring initial rates of oxygen consumption with oxygen electrode thermostated at, 0.2 mM oxygen in 50 mM sodium pyrophosphate, pH 8 | Arthrobacter globiformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | determined at 10, 25 and 40°C, 50 mM sodium pyrophosphate, potassium phosphate used for pH 7 and 7.5, measured at a fixed concentration of 0.2 mM oxygen and of 0.25 mM oxygen for the data at 25°C | Arthrobacter globiformis |
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Release 2023.1 (January 2023)
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