Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli strain Rosetta (DE3)pLysS | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
H99N | mutant, analysis of kinetic parameters | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | analysis of substrate kinetic isotope effects | Arthrobacter globiformis | |
1.3 | - |
choline | substrate choline, pH 7, 25°C | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | Q7X2H8 | - |
- |
Purification (Comment) | Organism |
---|---|
purified with the bound flavin cofactor in the fully oxidized state | Arthrobacter globiformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
0.28 | - |
apparent, H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
choline oxidase | - |
Arthrobacter globiformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
analysis of temperature effect on rate constant for flavin reduction | Arthrobacter globiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.03 | - |
choline | apparent, H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis | |
1.86 | - |
choline | H99N mutant, substrate choline, pH 7, 25°C. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | H99N mutant | Arthrobacter globiformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | H99N mutant, lower KM compared to pH 8-11. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
8 | 11 | H99N mutant, no impact on KM, highest KM. Measuring the rate of oxygen consumption with a computer-interfaced Oxy-32 oxygen monitoring system | Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arthrobacter globiformis |