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Literature summary for 1.1.3.18 extracted from

  • Shimao, M.; Tsuda, T.; Takahashi, M.; Kato, N.; Sakazawa, C.
    Purification of membrane-bound polyinyl alcohol oxidase in Pseudomonas sp. VM15C (1983), FEMS Microbiol. Lett., 20, 429-433.
No PubMed abstract available
Search for more literature for 1.1.3.18

Inhibitors

Inhibitors Comment Organism Structure
BaCl2
-
 Pseudomonas sp.
EDTA weak inhibition Pseudomonas sp.
HgCl2
-
 Pseudomonas sp.
MnCl2
-
 Pseudomonas sp.
NiCl2
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 polyvinyl alcohol
-
 Pseudomonas sp.
11
-
 4-heptanol
-
 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pseudomonas sp. 5829
-
extracellular
-
 Pseudomonas sp.
-
-
intracellular
-
 Pseudomonas sp. 5622
-
membrane membranous matrix Pseudomonas sp. 16020
-

Organism

Organism UniProt Comment Textmining
no activity in Pseudomonas putida
-
-
-
no activity in Pseudomonas putida VM15A
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp.
-
 symbiotic mixed culture of VM15C with Pseudomonas putida VM15A producing the growth factor A
-
Pseudomonas sp. VM15C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.38
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,6-hexanetriol + O2
-
 Pseudomonas sp. 1,2,6-hexanetrione + H2O2
-
 ?
2,5-hexanediol + O2
-
 Pseudomonas sp. 2,5-hexanedione + H2O2
-
 ?
2,5-hexanediol + O2
-
 Pseudomonas sp. VM15C 2,5-hexanedione + H2O2
-
 ?
2-hexanol + O2
-
 Pseudomonas sp. 2-hexanone + H2O2
-
 ?
2-hexanol + O2
-
 Pseudomonas sp. VM15C 2-hexanone + H2O2
-
 ?
2-propanol + O2
-
 Pseudomonas sp. 2-propanone + H2O2
-
 ?
2-propanol + O2
-
 Pseudomonas sp. VM15C 2-propanone + H2O2
-
 ?
3-pentanol + O2
-
 Pseudomonas sp. 3-pentanone + H2O2
-
 ?
4-heptanol + O2
-
 Pseudomonas sp. 4-heptanone + H2O2
-
 ?
4-heptanol + O2
-
 Pseudomonas sp. VM15C 4-heptanone + H2O2
-
 ?
polyvinyl alcohol + 2,6-dichlorophenolindophenol
-
 Pseudomonas sp. polyvinylketone + reduced 2,6-dichlorophenolindophenol
-
 ?
polyvinyl alcohol + ferricyanide
-
 Pseudomonas sp. polyvinylketone + ferrocyanide
-
 ?
polyvinyl alcohol + nitro blue tetrazolium
-
 Pseudomonas sp. polyvinylketone + reduced nitro blue tetrazolium
-
 ?
polyvinyl alcohol + O2
-
 Pseudomonas sp. polyvinylketone + H2O2
-
 ?
polyvinyl alcohol + O2
-
 Pseudomonas sp. VM15C polyvinylketone + H2O2
-
 ?
polyvinyl alcohol + phenazine ethosulfate
-
 Pseudomonas sp. polyvinylketone + reduced phenazine ethosulfate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.4
-
 Pseudomonas sp.
9.6
-
 purified enzyme Pseudomonas sp.