Literature summary for 1.1.3.19 extracted from

Bhat, S.G.; Vaidyanathan, C.S.
Purification and properties of L-4-hydroxymandelate oxidase from Pseudomonas convexa (1976), Eur. J. Biochem., 68, 323-331.

General Stability

General Stability	Organism
after solubilization the enzyme gradually loses activity when kept at 5°C, full reactivation by freezing and thawing	Pseudomonas putida

Inhibitors	Comment	Organism
2,4-Dinitrophenylhydrazine	-	Pseudomonas putida
2-mercaptoethanol	-	Pseudomonas putida
8-hydroxyquinoline	-	Pseudomonas putida
Ag+	-	Pseudomonas putida
Atebrin	-	Pseudomonas putida
Co2+	-	Pseudomonas putida
Cu2+	-	Pseudomonas putida
cysteine	-	Pseudomonas putida
diethyldithiocarbamate	-	Pseudomonas putida
dithiothreitol	-	Pseudomonas putida
DL-3,4-dihydroxymandelic acid	-	Pseudomonas putida
EDTA	-	Pseudomonas putida
Fe2+	-	Pseudomonas putida
Fe3+	-	Pseudomonas putida
guanidine hydrochloride	-	Pseudomonas putida
Hg2+	-	Pseudomonas putida
hydroxylamine	-	Pseudomonas putida
potassium cyanide	-	Pseudomonas putida
reduced glutathione	-	Pseudomonas putida
SDS	-	Pseudomonas putida
Zn2+	-	Pseudomonas putida

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.44	-	DL-4-hydroxymandelate	-	Pseudomonas putida

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	bound	Pseudomonas putida	16020	-

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Pseudomonas putida

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
155000	-	gel filtration	Pseudomonas putida

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2	Pseudomonas putida	enzyme of degradation pathway from mandelic acid to benzoic acid	4-hydroxybenzaldehyde + CO2 + H2O2	-	?

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Comment)	Organism
partial	Pseudomonas putida

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Pseudomonas putida

Storage Stability	Organism
-20°C, 2 months, solubilized enzyme	Pseudomonas putida
-20°C, particulate fraction suspended in 0.025 M sodium phosphate buffer, pH 7.0, more than 6 months	Pseudomonas putida

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2	absolute requirement for molecular oxygen, specific for L-isomer of 4-hydroxymandelic acid	Pseudomonas putida	4-hydroxybenzaldehyde + CO2 + H2O2	-	?
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2	enzyme of degradation pathway from mandelic acid to benzoic acid	Pseudomonas putida	4-hydroxybenzaldehyde + CO2 + H2O2	-	?
DL-4-hydroxymandelate + ?	-	Pseudomonas putida	?	-	?

Synonyms	Comment	Organism
L-4-hydroxymandelate oxidase (decarboxylating)	-	Pseudomonas putida

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Pseudomonas putida

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	-	-	Pseudomonas putida

Cofactor	Comment	Organism	Structure
FAD	required	Pseudomonas putida