Literature summary for 1.1.3.2 extracted from

Hiraka, K.; Kojima, K.; Lin, C.E.; Tsugawa, W.; Asano, R.; La Belle, J.T.; Sode, K.
Minimizing the effects of oxygen interference on l-lactate sensors by a single amino acid mutation in Aerococcus viridans L-lactate oxidase (2018), Biosens. Bioelectron., 103, 163-170 .

Search for more literature for 1.1.3.2

Application

Application	Comment	Organism
analysis	engineering the enzyme in order to minimize the effects of oxygen interference on sensor strips. Mutant A96L shows a drastic reduction in oxidase activity using molecular oxygen as the electron acceptor and a small increase in dehydrogenase activity employing an artificial electron acceptor. After immobilization on a screen-printed carbon electrode and under argon or atmospheric conditions, the response current increases linearly from 0.05 to 0.5 mM L-lactate for both wild-type and mutant A96L. Under atmospheric conditions, the response of wild-type electrode is suppressed by 9-12% due to oxygen interference. The mutant maintains 56-69% of the response current at the same L-lactate level and minimizes the relative bias error to -19% from -49% of wild-type	Aerococcus viridans

Protein Variants

Protein Variants	Comment	Organism
A96L	engineering the enzyme in order to minimize the effects of oxygen interference on sensor strips. Mutant A96L shows a drastic reduction in oxidase activity using molecular oxygen as the electron acceptor and a small increase in dehydrogenase activity employing an artificial electron acceptor. After immobilization on a screen-printed carbon electrode and under argon or atmospheric conditions, the response current increases linearly from 0.05 to 0.5 mM L-lactate for both wild-type and mutant A96L. Under atmospheric conditions, the response of wild-type electrode is suppressed by 9-12% due to oxygen interference. The mutant maintains 56-69% of the response current at the same L-lactate level and minimizes the relative bias error to -19% from -49% of wild-type	Aerococcus viridans

Organism

Organism	UniProt	Comment	Textmining
Aerococcus viridans	Q44467	-	-

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Release 2023.1 (January 2023)