Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.2 extracted from

  • Stoisser, T.; Rainer, D.; Leitgeb, S.; Wilson, D.K.; Nidetzky, B.
    The Ala95-to-Gly substitution in Aerococcus viridans l-lactate oxidase revisited - structural consequences at the catalytic site and effect on reactivity with O2 and other electron acceptors (2015), FEBS J., 282, 562-578 .
    View publication on PubMed
Search for more literature for 1.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aerococcus viridans

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant A95G in complex with pyruvate, to 1.65 A resolution. Mutation leads to increased steric volume available in the active site Aerococcus viridans

Protein Variants

Protein Variants Comment Organism
A95G mutant is 3fold more reactive towards 2,6-dichlorophenol-indophenol than O2, whereas wildtype is 14fold more reactive towards O2 than 2,6-dichlorophenol-indophenol. Substituted 1,4-benzoquinones are up to 5fold better electron acceptors for reaction with L-lactate-reduced A95G variant than wild-type Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information detailed analysis of reductive and oxidative half-reaction Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans Q44467
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-hydroxybutanoate + O2
-
 Aerococcus viridans 2-oxobutanoate + H2O2
-
 ?
(S)-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?
(S)-mandelic acid + O2
-
 Aerococcus viridans phenylpyruvate + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
140
-
 (S)-lactate mutant A95G, pH 6.5, 20°C Aerococcus viridans
140
-
 (S)-lactate wild-type, pH 6.5, 20°C Aerococcus viridans