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Literature summary for 1.1.3.2 extracted from

  • Hackenberg, C.; Kern, R.; Huege, J.; Stal, L.J.; Tsuji, Y.; Kopka, J.; Shiraiwa, Y.; Bauwe, H.; Hagemann, M.
    Cyanobacterial lactate oxidases serve as essential partners in N2 fixation and evolved into photorespiratory glycolate oxidases in plants (2011), Plant Cell, 23, 2978-2990 .
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
F212V change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 25fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418
L112W change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 2fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418
M82T change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 10fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418
M82T/L112W/F212V change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, reverse the L-lactate oxidase/glycolate oxidase activity ratio Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 L-lactate pH 8.0, 30°C Nostoc sp. PCC 7120 = FACHB-418
0.081
-
 L-lactate pH 8.0, 30°C Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii F8WQN2 cf. EC 1.1.3.1, enzyme shows high L-lactate oxidase and low glycolate oxidase activities
-
Nostoc sp. PCC 7120 = FACHB-418 Q8Z0C8 cf. EC 1.1.3.1, enzyme shows high L-lactate oxidase and low glycolate oxidase activities
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerate + O2 5.9% of the activity with L-lactate Nostoc sp. PCC 7120 = FACHB-418 hydroxypyruvate + H2O2
-
 ?
glyoxylate + O2 15.3% of the activity with L-lactate Nostoc sp. PCC 7120 = FACHB-418 oxalate + H2O2
-
 ?
L-lactate + O2
-
 Nostoc sp. PCC 7120 = FACHB-418 pyruvate + H2O2
-
 ?
L-lactate + O2
-
 Chlamydomonas reinhardtii pyruvate + H2O2
-
 ?
additional information no substrate: D-lactate. Poor substrate: glycolate Nostoc sp. PCC 7120 = FACHB-418 ?
-
 ?
additional information no substrate: D-lactate. Poor substrate: glycolate Chlamydomonas reinhardtii ?
-
 ?

General Information

General Information Comment Organism
physiological function overexpression decreases the accumulation of toxic glycolate in a cyanobacterial photorespiratory mutant and restores its ability to grow in air. A lactate oxidase-deficient Nostoc sp. mutant grows normally in nitrate-containing medium but dies under N2-fixing conditions. The enzyme may primarily serve as an O2-scavenging enzyme to protect nitrogenase in extant N2-fixing cyanobacteria Nostoc sp. PCC 7120 = FACHB-418