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Literature summary for 1.1.3.20 extracted from

  • Banthorpe, D.V.; Cardemil, E.; Del Carmen Contraras, M.
    Purification and properties of alcohole oxidase from Tanacetum vulgare (1976), Phytochemistry, 15, 391-394.
No PubMed abstract available

General Stability

General Stability Organism
prolonged dialysis against EDTA, stable to Tanacetum vulgare

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibitor: cyanide; no inhibitors: iodoacetate, MnSO4, EDTA, p-hydroxymercuribenzoate, 2,2'-dipyridyl, 1,10-phenanthroline, 8-hydroxyquinoline, fluoride, azide Tanacetum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
Hex-trans-2-ene-1-ol
-
Tanacetum vulgare
0.49
-
n-Octanol value above Tanacetum vulgare
1.56
-
geraniol
-
Tanacetum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare
94000
-
1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare
180000
-
gel filtration Tanacetum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
long-chain alcohol + O2 Tanacetum vulgare substrates are primary alcohols with a chain-length of C6-C10 ?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone stable to Tanacetum vulgare

Organism

Organism UniProt Comment Textmining
Tanacetum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Tanacetum vulgare

Reaction

Reaction Comment Organism Reaction ID
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2 Tanacetum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Tanacetum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
2.6
-
-
Tanacetum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geraniol + O2 i.e. 3,7-dimethylocta-trans-2,6-dien-1-ol, better substrate than cis-isomer, the 2-ene-group enhances activity Tanacetum vulgare 3,7-dimethylocta-trans-2,6-dien-1-al + H2O2
-
?
hex-trans-2-ene-1-ol + O2 preferred substrate, the 2-ene-group enhances activity Tanacetum vulgare hex-trans-2-ene-1-al + H2O2
-
?
long-chain alcohol + O2 substrates are primary alcohols with a chain-length of C6-C10 Tanacetum vulgare ?
-
?
additional information substrates are primary alcohols with a chain-length of C6-C10, optimal chain-length: C8-C10 Tanacetum vulgare ?
-
?
additional information no substrates for Tanacetum enzyme: secondary, tertiary alcohols and Tris Tanacetum vulgare ?
-
?
n-octanol + O2
-
Tanacetum vulgare n-octanal + H2O2
-
?
terpenol + O2 poor substrate Tanacetum vulgare ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
18 22
-
Tanacetum vulgare

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
-
45 about 60% of maximal activity at 0┬░C and about 30% of maximal activity at 45┬░C Tanacetum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 9 plateau Tanacetum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
4.8 10 about half-maximal activity at pH 4.8 and 10.0 Tanacetum vulgare

Cofactor

Cofactor Comment Organism Structure
additional information NAD(P)+ Tanacetum vulgare
additional information FAD, FMN Tanacetum vulgare