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Literature summary for 1.1.3.20 extracted from

  • Dickinson, F.M.; Wadforth, C.
    Purification and some properties of alcohol oxidase from alkane-grown Candida tropicalis (1992), Biochem. J., 282, 325-331.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Detergents e.g. cholate, activation, purified preparations do not need detergents for activation Candida tropicalis

General Stability

General Stability Organism
CHAPS, 0.5%, stabilizes during solubilization Candida tropicalis
cholate, 1%, solubilizes and stabilizes Candida tropicalis
phenylmethylsulfonyl fluoride stabilizes during purification and storage Candida tropicalis

Inhibitors

Inhibitors Comment Organism Structure
light complete inactivation, t1/2: 7 min; photochemical inactivation at wavelength of 450 nm, i.e. blue-light region of the spectrum of visible light Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
Tetradecanol
-
Candida tropicalis
0.005
-
hexadecanol
-
Candida tropicalis
0.0061
-
Dodecanol
-
Candida tropicalis
0.042
-
Decanol
-
Candida tropicalis
0.061
-
O2
-
Candida tropicalis
1
-
Octanol
-
Candida tropicalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Candida tropicalis 16020
-
microsome
-
Candida tropicalis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000, SDS-PAGE Candida tropicalis
145000
-
gel filtration Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
long-chain alcohol + O2 Candida tropicalis
-
?
-
?

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida tropicalis

Reaction

Reaction Comment Organism Reaction ID
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 ping-pong mechanism Candida tropicalis
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2 Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
30
-
-
Candida tropicalis

Storage Stability

Storage Stability Organism
0┬░C, in the dark stable for at least 10 h Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-tetradecanol + O2 75% the rate of dodecanol oxidation Candida tropicalis tetradecanal + H2O2
-
?
12-hydroxydodecanoic acid + O2 poor substrate Candida tropicalis ?
-
?
2-dodecanol + O2
-
Candida tropicalis ?
-
?
alpha,omega-alkanediols + O2 from C10 to C16 Candida tropicalis ?
-
?
decanol + O2 oxidation at 70% the rate of dodecanol oxidation Candida tropicalis decanal + H2O2
-
?
dodecanol + O2 i.e. lauryl alcohol, preferred substrate Candida tropicalis dodecanal + H2O2
-
?
hexadecanol + O2 19% the rate of dodecanol oxidation Candida tropicalis hexadecanal + H2O2
-
?
long-chain alcohol + O2
-
Candida tropicalis ?
-
?
additional information weak activity towards fatty aldehydes Candida tropicalis ?
-
?
additional information no substrate: dodecan-4-ol, dodecan-5-ol Candida tropicalis ?
-
?
n-octanol + O2 oxidation at 34% the rate of dodecanol oxidation Candida tropicalis n-octanal + H2O2
-
?
octadecanol + O2 oxidation at 12% the rate of dodecanol oxidation Candida tropicalis octadecanal + H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Candida tropicalis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
25
-
inactivation overnight Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
60% activity at pH 7.0 Candida tropicalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
inactivation after 3 min Candida tropicalis
7
-
and below unstable Candida tropicalis
9
-
at least 15 min stable Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
flavin not FAD, FMN, riboflavin, different absorption and fluorescence emission spectra, flavin is probably covalently bound Candida tropicalis
additional information no cofactor: riboflavin, 2,6-dichlorophenolindophenol/phenazine methosulfate, potassium ferricyanide, cytochrome c, nitro blue tetrazolium, tetramethyl-p-phenylenediamine Candida tropicalis