Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Detergents | e.g. cholate, activation, purified preparations do not need detergents for activation | Candida tropicalis |
General Stability | Organism |
---|---|
CHAPS, 0.5%, stabilizes during solubilization | Candida tropicalis |
cholate, 1%, solubilizes and stabilizes | Candida tropicalis |
phenylmethylsulfonyl fluoride stabilizes during purification and storage | Candida tropicalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
light | complete inactivation, t1/2: 7 min; photochemical inactivation at wavelength of 450 nm, i.e. blue-light region of the spectrum of visible light | Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
Tetradecanol | - |
Candida tropicalis | |
0.005 | - |
hexadecanol | - |
Candida tropicalis | |
0.0061 | - |
Dodecanol | - |
Candida tropicalis | |
0.042 | - |
Decanol | - |
Candida tropicalis | |
0.061 | - |
O2 | - |
Candida tropicalis | |
1 | - |
Octanol | - |
Candida tropicalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Candida tropicalis | 16020 | - |
microsome | - |
Candida tropicalis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Candida tropicalis |
145000 | - |
gel filtration | Candida tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
long-chain alcohol + O2 | Candida tropicalis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida tropicalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 | ping-pong mechanism | Candida tropicalis | |
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 | 2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2 | Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Candida tropicalis |
Storage Stability | Organism |
---|---|
0°C, in the dark stable for at least 10 h | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-tetradecanol + O2 | 75% the rate of dodecanol oxidation | Candida tropicalis | tetradecanal + H2O2 | - |
? | |
12-hydroxydodecanoic acid + O2 | poor substrate | Candida tropicalis | ? | - |
? | |
2-dodecanol + O2 | - |
Candida tropicalis | ? | - |
? | |
alpha,omega-alkanediols + O2 | from C10 to C16 | Candida tropicalis | ? | - |
? | |
decanol + O2 | oxidation at 70% the rate of dodecanol oxidation | Candida tropicalis | decanal + H2O2 | - |
? | |
dodecanol + O2 | i.e. lauryl alcohol, preferred substrate | Candida tropicalis | dodecanal + H2O2 | - |
? | |
hexadecanol + O2 | 19% the rate of dodecanol oxidation | Candida tropicalis | hexadecanal + H2O2 | - |
? | |
long-chain alcohol + O2 | - |
Candida tropicalis | ? | - |
? | |
additional information | weak activity towards fatty aldehydes | Candida tropicalis | ? | - |
? | |
additional information | no substrate: dodecan-4-ol, dodecan-5-ol | Candida tropicalis | ? | - |
? | |
n-octanol + O2 | oxidation at 34% the rate of dodecanol oxidation | Candida tropicalis | n-octanal + H2O2 | - |
? | |
octadecanol + O2 | oxidation at 12% the rate of dodecanol oxidation | Candida tropicalis | octadecanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000, SDS-PAGE | Candida tropicalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
inactivation overnight | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
60% activity at pH 7.0 | Candida tropicalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
inactivation after 3 min | Candida tropicalis |
7 | - |
and below unstable | Candida tropicalis |
9 | - |
at least 15 min stable | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | not FAD, FMN, riboflavin, different absorption and fluorescence emission spectra, flavin is probably covalently bound | Candida tropicalis | |
additional information | no cofactor: riboflavin, 2,6-dichlorophenolindophenol/phenazine methosulfate, potassium ferricyanide, cytochrome c, nitro blue tetrazolium, tetramethyl-p-phenylenediamine | Candida tropicalis |