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Literature summary for 1.1.3.20 extracted from

  • Zhao, S.; Lin, Z.; Ma, W.; Luo, D.; Cheng, Q.
    Cloning and characterization of long-chain fatty alcohol oxidase LjFAO1 in lotus japonicus (2008), Biotechnol. Prog., 24, 773-779.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full length cDNA and C-terminal deleted protein overexpressed in Escherichia coli BL21(DE3), pET-28a and pGEM-T easy plasmids Lotus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
1,16-hexadecanediol 1 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana Lotus japonicus
0.049
-
1-hexadecanol 1 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana Lotus japonicus
0.059
-
1-Dodecanol 5 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana Lotus japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
long-chain alcohol + O2 Lotus japonicus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Lotus japonicus
-
ecotype Gifu B-129
-

Purification (Commentary)

Purification (Comment) Organism
full length version of LjFAO1 protein, gel purification Lotus japonicus

Source Tissue

Source Tissue Comment Organism Textmining
seedling 8-day-old seedlings, RT-PCR, whole plant expression shown, cold stress treatment of Lotus japonicus
-
silique lowest mRNA expression in Lotus japonicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
LjFAO1 gene identified, genomic structure and mRNA expression, cold stress profiling of LjFAO1 transcripts, overexpression of full-lenght and C-truncated cDNAs, full-length version but not C-truncated part of overexpressed LjFAO1-gene shows long-chain fatty alcohol oxidase activity, substrate specificites of purified protein shown Lotus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,16-hexadecanediol + O2 purified active LjFAO1 protein Lotus japonicus ?
-
?
1-dodecanol + O2 purified active LjFAO1 protein Lotus japonicus dodecanal + H2O2
-
?
1-hexadecanol + O2 purified active LjFAO1 protein Lotus japonicus hexadecanal + H2O2
-
?
long-chain alcohol + O2
-
Lotus japonicus ?
-
?

Synonyms

Synonyms Comment Organism
LjFAO1 protein
-
Lotus japonicus