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Literature summary for 1.1.3.20 extracted from

  • Rembeza, E.; Boverio, A.; Fraaije, M.W.; Engqvist, M.K.M.
    Discovery of two novel oxidases using a high-throughput activity screen (2022), Chembiochem, 23, e202100510 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0277
-
1-Dodecanol pH 7.4, 25┬░C uncultured marine group II/III euryarchaeote KM3_72_H01

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane sequence contains a predicted membrane binding helix within the substrate binding domain uncultured marine group II/III euryarchaeote KM3_72_H01 16020
-

Organism

Organism UniProt Comment Textmining
uncultured marine group II/III euryarchaeote KM3_72_H01 A0A075HNX4
-
-

Renatured (Commentary)

Renatured (Comment) Organism
enzyme is able to refold and regain its activity upon heating up to 65┬░C uncultured marine group II/III euryarchaeote KM3_72_H01

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.23
-
substrate 1-dodecanol, pH 7.4, 25┬░C uncultured marine group II/III euryarchaeote KM3_72_H01

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-decanol + O2
-
uncultured marine group II/III euryarchaeote KM3_72_H01 decanal + H2O2
-
?
1-dodecanol + O2
-
uncultured marine group II/III euryarchaeote KM3_72_H01 dodecanal + H2O2
-
?
1-octanol + O2
-
uncultured marine group II/III euryarchaeote KM3_72_H01 octanal + H2O2
-
?
1-tetradecanol + O2
-
uncultured marine group II/III euryarchaeote KM3_72_H01 tetradecanal + H2O2
-
?
additional information enzyme is active with substrates having a carbon chain length of six and above, showing the highest activity with 1-dodecanol uncultured marine group II/III euryarchaeote KM3_72_H01 ?
-
-

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
80
-
30 min, complete loss of activity uncultured marine group II/III euryarchaeote KM3_72_H01

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
uncultured marine group II/III euryarchaeote KM3_72_H01

pH Range

pH Minimum pH Maximum Comment Organism
5
-
almost complete loss of activity uncultured marine group II/III euryarchaeote KM3_72_H01