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Literature summary for 1.1.3.29 extracted from

  • Rembeza, E.; Boverio, A.; Fraaije, M.W.; Engqvist, M.K.M.
    Discovery of two novel oxidases using a high-throughput activity screen (2022), Chembiochem, 23, e202100510 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.29

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ralstonia solanacearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 N-acetyl-beta-D-glucosamine pH 7.4, 25°C Ralstonia solanacearum
0.32
-
 N-acetyl-D-galactosamine pH 7.4, 25°C Ralstonia solanacearum
1.4
-
 D-galactosamine pH 7.4, 25°C Ralstonia solanacearum
4.5
-
 D-glucosamine pH 7.4, 25°C Ralstonia solanacearum
19
-
 chitobiose pH 7.4, 25°C Ralstonia solanacearum
65
-
 D-mannosamine pH 7.4, 25°C Ralstonia solanacearum
102
-
 D-galactose pH 7.4, 25°C Ralstonia solanacearum
118
-
 D-mannose pH 7.4, 25°C Ralstonia solanacearum
182
-
 N-acetyl-D-mannosamine pH 7.4, 25°C Ralstonia solanacearum
216
-
 D-glucose pH 7.4, 25°C Ralstonia solanacearum

Organism

Organism UniProt Comment Textmining
Ralstonia solanacearum A3RXB7
-
-
Ralstonia solanacearum UW551 A3RXB7
-
-

Renatured (Commentary)

Renatured (Comment) Organism
enzyme is able to refold and regain its activity upon heating to up to 80°C Ralstonia solanacearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitobiose + O2
-
 Ralstonia solanacearum ?
-
 ?
D-galactosamine + O2
-
 Ralstonia solanacearum ?
-
 ?
D-galactosamine + O2
-
 Ralstonia solanacearum UW551 ?
-
 ?
D-galactose + O2
-
 Ralstonia solanacearum ?
-
 ?
D-glucosamine + O2
-
 Ralstonia solanacearum ?
-
 ?
D-glucosamine + O2
-
 Ralstonia solanacearum UW551 ?
-
 ?
D-glucose + O2
-
 Ralstonia solanacearum ?
-
 ?
D-mannosamine + O2
-
 Ralstonia solanacearum ?
-
 ?
D-mannose + O2
-
 Ralstonia solanacearum ?
-
 ?
N-acetyl-beta-D-glucosamine + O2
-
 Ralstonia solanacearum N-acetyl-D-glucosaminolactone + H2O2
-
 ?
N-acetyl-beta-D-glucosamine + O2
-
 Ralstonia solanacearum UW551 N-acetyl-D-glucosaminolactone + H2O2
-
 ?
N-acetyl-D-galactosamine + O2
-
 Ralstonia solanacearum N-acetyl-D-galactosaminolactone + H2O2
-
 ?
N-acetyl-D-galactosamine + O2
-
 Ralstonia solanacearum UW551 N-acetyl-D-galactosaminolactone + H2O2
-
 ?
N-acetyl-D-mannosamine + O2
-
 Ralstonia solanacearum N-acetyl-D-mannosaminolactone + H2O2
-
 ?
N-acetyl-D-mannosamine + O2
-
 Ralstonia solanacearum UW551 N-acetyl-D-mannosaminolactone + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
RRSL_02030
-
 Ralstonia solanacearum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
39
-
 melting temperature, in buffers with pH 6.5 and above Ralstonia solanacearum

Cofactor

Cofactor Comment Organism Structure
FAD absorption spectrum displays maxima at 375 and 445 nm, FAD is bound to residues His64 and Cys123 Ralstonia solanacearum