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Literature summary for 1.1.3.38 extracted from

  • van den Heuvel, R.H.; Fraaije, M.W.; Mattevi, A.; van Berkel, W.J.
    Asp-170 is crucial for the redox properties of vanillyl-alcohol oxidase (2000), J. Biol. Chem., 275, 14799-14808.
    View publication on PubMed
Search for more literature for 1.1.3.38

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of mutant enzyme D170S are grown using hanging-drop vapor diffusion method Penicillium simplicissimum

Protein Variants

Protein Variants Comment Organism
D170A 3100fold decrease in turnover-number for for 4-(methoxymethyl)phenol Penicillium simplicissimum
D170E 2fold increase in KM-value for for 4-(methoxymethyl)phenol, 2.1fold increase in Km-value for vanillyl alcohol, 2fold decrease in KM-value for eugenol as compared to wild-type enzyme.442fold decrease in turnover-number for for 4-(methoxymethyl)phenol, 2.5fold decrease in turnover-number for vanillyl alcohol, 51.5fold decrease in turnover-number for eugenol as compared to wild-type enzyme.Redox potential of mutant enzyme, + 6 mV, is decreased compared to wild-type enzyme, 55 mV Penicillium simplicissimum
D170N 1550fold decrease in turnover-number for for 4-(methoxymethyl)phenol Penicillium simplicissimum
D170S 2fold decrease in KM-value for eugenol as compared to wild-type enzyme.1290fold decrease in turnover-number for for 4-(methoxymethyl)phenol, 825fold decrease in turnover-number for vanillyl alcohol, 1750fold decrease in turnover-number for eugenol as compared to wild-type enzyme. Redox potential of mutant enzyme, -91 mV, is decreased compared to wild-type enzyme, 55 mV Penicillium simplicissimum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 eugenol pH 7.5, 25°C, mutant enzyme D170E Penicillium simplicissimum
0.002
-
 eugenol pH 7.5, 25°C, mutant enzyme D170S Penicillium simplicissimum
0.113
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170E Penicillium simplicissimum
0.34
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme D170E Penicillium simplicissimum

Organism

Organism UniProt Comment Textmining
Penicillium simplicissimum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium simplicissimum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(methoxymethyl)phenol + O2
-
 Penicillium simplicissimum ?
-
 ?
eugenol + O2
-
 Penicillium simplicissimum ?
-
 ?
vanillyl alcohol + O2
-
 Penicillium simplicissimum ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170N Penicillium simplicissimum
0.004
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170S Penicillium simplicissimum
0.004
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme D170S Penicillium simplicissimum
0.01
-
 eugenol pH 7.5, 25°C, mutant enzyme D170S Penicillium simplicissimum
0.07
-
 4-(Methoxymethyl)phenol pH 7.5, 25°C, mutant enzyme D170E Penicillium simplicissimum
0.34
-
 eugenol pH 7.5, 25°C, mutant enzyme D170E Penicillium simplicissimum
1.3
-
 Vanillyl alcohol pH 7.5, 25°C, mutant enzyme D170E Penicillium simplicissimum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Penicillium simplicissimum