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Literature summary for 1.1.3.38 extracted from
- A single loop is essential for the octamerization of vanillyl alcohol oxidase (2016), FEBS J., 283, 2546-2559.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Penicillium simplicissimum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0039 | - |
eugenol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum |  |
| 0.012 | - |
4-Ethylphenol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
| 0.149 | - |
Vanillyl alcohol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
8 * 61000, SDS-PAGE | Penicillium simplicissimum |
| 64000 | - |
- |
Penicillium simplicissimum |
| 510000 | - |
gel filtration | Penicillium simplicissimum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| vanillyl alcohol + O2 | Penicillium simplicissimum | - |
vanillin + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium simplicissimum | P56216 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl Sepharose column chromatography, and hydroxyapatite column chromatography | Penicillium simplicissimum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-ethylphenol + O2 + H2O | - |
Penicillium simplicissimum | ? + H2O2 | - |
? | |
| eugenol + O2 + H2O | - |
Penicillium simplicissimum | coniferyl alcohol + H2O2 | - |
? | |
| vanillyl alcohol + O2 | - |
Penicillium simplicissimum | vanillin + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homooctamer | 8 * 61000, SDS-PAGE | Penicillium simplicissimum |
| homooctamer | 8 * 64000, calculated from amino acid sequence | Penicillium simplicissimum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VAO | - |
Penicillium simplicissimum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 54.6 | - |
melting temperature | Penicillium simplicissimum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
Vanillyl alcohol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
| 2.6 | - |
4-Ethylphenol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
| 11 | - |
eugenol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Penicillium simplicissimum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17 | - |
Vanillyl alcohol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
| 217 | - |
4-Ethylphenol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
| 2821 | - |
eugenol | wild type enzyme, at pH 7.5 and 25°C | Penicillium simplicissimum | |
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