Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodococcus jostii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
4-ethylguaiacol | pH 7.5, 25°C | Rhodococcus jostii | |
0.0023 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C | Rhodococcus jostii | |
0.04 | - |
Vanillyl alcohol | pH 7.5, 25°C | Rhodococcus jostii | |
0.076 | - |
vanillylamine | pH 7.5, 25°C | Rhodococcus jostii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
111000 | - |
gel filtration | Rhodococcus jostii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | Q0SBK1 | - |
- |
Purification (Comment) | Organism |
---|---|
recpombinant enzyme | Rhodococcus jostii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(methoxymethyl)phenol + O2 | - |
Rhodococcus jostii | ? + H2O2 | - |
? | |
4-ethylguaiacol + O2 | - |
Rhodococcus jostii | ? + H2O2 | - |
? | |
5-indanol + O2 | - |
Rhodococcus jostii | ? + H2O2 | - |
? | |
additional information | enzyme also oxidizes eugenol. Poor substrates: vanillylamine, 4-ethylguaiacol | Rhodococcus jostii | ? | - |
- |
|
vanillyl alcohol + O2 | - |
Rhodococcus jostii | vanillin + H2O2 | - |
? | |
vanillylamine + O2 | - |
Rhodococcus jostii | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE, 2 * 58681, calculated from sequence | Rhodococcus jostii |
Synonyms | Comment | Organism |
---|---|---|
RHA1_ro03282 | - |
Rhodococcus jostii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
0.0034 mM enzyme in in 20 mm Tris ? HCl, pH 7.5 for 90 min, no inactivation | Rhodococcus jostii |
60 | - |
half-life 30 min, in presence of 3fold excess of FAD half-life 45 min | Rhodococcus jostii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C | Rhodococcus jostii | |
0.026 | - |
4-ethylguaiacol | pH 7.5, 25°C | Rhodococcus jostii | |
0.26 | - |
vanillylamine | pH 7.5, 25°C | Rhodococcus jostii | |
12 | - |
Vanillyl alcohol | pH 7.5, 25°C | Rhodococcus jostii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | more than 90% of maximum activity | Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | eugenol oxidase is partly expressed in the apo form, but can be fully flavinylated by the addition of FAD. FAD is covalently linked to residue H390. Protein shows absorption maxima at 365 nm and 441 nm, and shoulders at 313 nm, 394 nm, and 461 nm | Rhodococcus jostii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C | Rhodococcus jostii | |
3.4 | - |
vanillylamine | pH 7.5, 25°C | Rhodococcus jostii | |
12 | - |
4-ethylguaiacol | pH 7.5, 25°C | Rhodococcus jostii | |
300 | - |
Vanillyl alcohol | pH 7.5, 25°C | Rhodococcus jostii |