Protein Variants | Comment | Organism |
---|---|---|
additional information | preparation of surface variants that contain artificial polymer poylethylene glycol. All surface modifications of glucose oxidase beyond that of the wild-type enzyme give rise to altered behavior for hydrogen transfer in the active site such that the kinetic isotope effect becomes more temperature-dependent upon perturbation | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
136000 | - |
x * 136000 deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus niger |
146000 | - |
x * 146000, deglycosylated recombinant enzyme with PEG-350 reagent, SDS-PAGE | Aspergillus niger |
155000 | - |
x * 155000, wild-type enzyme, SDS-PAGE | Aspergillus niger |
211000 | - |
x * 211000, deglycosylated recombinant enzyme with PEG-5000 reagent, SDS-PAGE | Aspergillus niger |
320000 | - |
x * 320000, recombinant enzyme expressed in yeast, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 136000 deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus niger |
? | x * 146000, deglycosylated recombinant enzyme with PEG-350 reagent, SDS-PAGE | Aspergillus niger |
? | x * 155000, wild-type enzyme, SDS-PAGE | Aspergillus niger |
? | x * 211000, deglycosylated recombinant enzyme with PEG-5000 reagent, SDS-PAGE | Aspergillus niger |
? | x * 320000, recombinant enzyme expressed in yeast, SDS-PAGE | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
beta-D-glucose | pH 9.0, 33°C, deglycosylated recombinant enzyme with PEG-5000 reagent | Aspergillus niger | |
5.5 | - |
beta-D-glucose | pH 9.0, 33°C, deglycosylated recombinant enzyme with PEG-350 reagent | Aspergillus niger | |
5.9 | - |
beta-D-glucose | pH 9.0, 33°C, recombinant enzyme expressed in yeast | Aspergillus niger | |
6.1 | - |
beta-D-glucose | pH 9.0, 33°C, wild-type enzyme | Aspergillus niger | |
6.3 | - |
beta-D-glucose | pH 9.0, 33°C, deglycosylated recombinant enzyme | Aspergillus niger |