Application | Comment | Organism |
---|---|---|
synthesis | utilization of recombinant enzyme expressed in the periplasm or on the cell surface of Escherichia coli as biocatalyst in a non-laborious and non-costly whole-cell application for reacting on towards different polyols such as xylitol and sorbitol | Streptomyces coelicolor |
Cloned (Comment) | Organism |
---|---|
recombinant enzyme expression in the periplasm or on the cell surface of Escherichia coli cells of different strains. The enzyme is differently tagged, i.e. expressed as Tat-AldO or INP-AldO, and exported to the periplasm or to the cell surface of the transformed cells, overview. AldO is successfully displayed at the surface of E. coli using a truncated INP variant, it contains covalently bound FAD, thus has attained a correctly folded and active conformation | Streptomyces coelicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Streptomyces coelicolor | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45100 | - |
1 * 45100 | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sorbitol + O2 | Streptomyces coelicolor | - |
? | - |
? | |
sorbitol + O2 | Streptomyces coelicolor A3(2) | - |
? | - |
? | |
xylitol + O2 | Streptomyces coelicolor | - |
? | - |
? | |
xylitol + O2 | Streptomyces coelicolor A3(2) | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AldO catalyzes the C1 oxidation of several polyols | Streptomyces coelicolor | ? | - |
? | |
additional information | AldO catalyzes the C1 oxidation of several polyols | Streptomyces coelicolor A3(2) | ? | - |
? | |
sorbitol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
sorbitol + O2 | - |
Streptomyces coelicolor A3(2) | ? | - |
? | |
xylitol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
xylitol + O2 | - |
Streptomyces coelicolor A3(2) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45100 | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
AldO | - |
Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | a flavoprotein, the flavin cofactor is covalently linked to the polypeptide chain, covalent anchoring of the FAD cofactor is an autocatalytic process and that only occurs upon correct folding of the polypeptide chain | Streptomyces coelicolor |