Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
synthesis | synthesis of rare sugars using Escherichia coli whole cells. The donor substrate dihydroxyacetone phosphate (DHAP) is generated from glycerol by glycerol kinase (GK) and glycerol phosphate oxidase (GPO). The acceptor D-glyceraldehyde is directly produced from glycerol by alditol oxidase. The aldol reaction between DHAP and D-glyceraldehyde is performed by L-rhamnulose-1-phosphate aldolase (RhaD) to generate the corresponding sugar-1-phosphate. Finally, the phosphate group is removed by fructose-1-phosphatase (YqaB) to obtain the rare sugars D-sorbose and D-psicose. Under the optimized conditions, the cascade produces 7.9 g/l of D-sorbose and D-psicose with a total conversion rate of 17.7% from glycerol | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + O2 | - |
Streptomyces coelicolor | D-glyceraldehyde + H2O2 | - |
? | |
glycerol + O2 | - |
Streptomyces coelicolor A3(2) | D-glyceraldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AldO | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces coelicolor |