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Literature summary for 1.1.3.43 extracted from

  • Huang, F.; Spiteller, D.; Koorbanally, N.A.; Li, Y.; Llewellyn, N.M.; Spencer, J.B.
    Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin (2007), ChemBioChem, 8, 283-288.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60034
-
x * 60034, LC-ESI-MS Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae Q53U15
-
-
Streptomyces fradiae NCIMB 8233 Q53U15
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase Streptomyces fradiae ?
-
?
additional information enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase Streptomyces fradiae NCIMB 8233 ?
-
?
paromamine + O2 oxygen-dependent mechanism for the regeneration of the FAD cofactor Streptomyces fradiae 6'-dehydro-paromamine + H2O2
-
?
paromamine + O2 oxygen-dependent mechanism for the regeneration of the FAD cofactor Streptomyces fradiae NCIMB 8233 6'-dehydro-paromamine + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 60034, LC-ESI-MS Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces fradiae

General Information

General Information Comment Organism
physiological function joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5’-phosphate-mediated transamination Streptomyces fradiae