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Literature summary for 1.1.3.47 extracted from

  • Mathieu, Y.; Offen, W.; Forget, S.; Ciano, L.; Viborg, A.; Blagova, E.; Henrissat, B.; Walton, P.; Davies, G.; Brumer, H.
    Discovery of a fungal copper radical oxidase with high catalytic efficiency toward 5-hydroxymethylfurfural and benzyl alcohols for bioprocessing (2020), ACS Catal., 10, 3042-3058 .
No PubMed abstract available
Search for more literature for 1.1.3.47

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Colletotrichum graminicola

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. The central, seven-bladed beta-propeller domain and the C-terminal, immunoglobulin-like beta-sandwich together constitute the AA5_2 catalytic module (residues Val199 to Leu689). The N-terminal domain contains conserved disulfide bridges and an overall fold typical of a PAN_1 domain Colletotrichum graminicola

Protein Variants

Protein Variants Comment Organism
Y334F mutant exhibits specific activities comparable to the wild-type for carbohydrates, diols, aryl alcohols, 5-hydroxymethylfurfural and 5-hydroxymethyl-2-furancarboxylic acid Colletotrichum graminicola
Y334W mutant exhibits increased specific activity toward carbohydrates and decreased activity toward diols, aryl alcohols, and furans Colletotrichum graminicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5
-
 5-hydroxymethylfurfural wild-type, pH 7,25°C Colletotrichum graminicola
14
-
 5-hydroxymethylfurfural mutant Y334F, pH 7,25°C Colletotrichum graminicola
26.9
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7,25°C Colletotrichum graminicola
27
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7,25°C Colletotrichum graminicola
42
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7,25°C Colletotrichum graminicola
53
-
 5-hydroxymethylfurfural mutant Y334W, pH 7,25°C Colletotrichum graminicola

Metals/Ions

Metals/Ions Comment Organism Structure
copper enzyme shows a copper-to-protein ratio of 1.08 Colletotrichum graminicola

Organism

Organism UniProt Comment Textmining
Colletotrichum graminicola E3Q9X3 cf. EC 1.1.3.9
-
Colletotrichum graminicola M1.001 E3Q9X3 cf. EC 1.1.3.9
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein protein is predicted to be N-glycosylated on sites N309, N386, and N644 Colletotrichum graminicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-(hydroxymethyl)furan-2-carboxylic acid + O2
-
 Colletotrichum graminicola 2,5-formylfurancarboxylic acid + H2O2
-
 ?
5-hydroxymethylfurfural + O2
-
 Colletotrichum graminicola 5-(hydroxymethyl)furan-2-carboxylic acid + H2O2
-
 ?
additional information enzyme is active on short-chain alkane diols and glycerol, and aryl alcohols, with similar specific activity values. Highest specific activity is observed on 5-hydroxymethylfurfural. Galactose and galactosylated oligosaccharides are poor substrates Colletotrichum graminicola ?
-
-

Subunits

Subunits Comment Organism
? x * 77500, SDS-PAGE, and x * 77507, calculated from sequence, deglycosylated protein Colletotrichum graminicola

Synonyms

Synonyms Comment Organism
GLRG_02805
-
 Colletotrichum graminicola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 initial loss of 10% of activity, thereafter stable or at least 22 h Colletotrichum graminicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7,25°C Colletotrichum graminicola
15.4
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7,25°C Colletotrichum graminicola
28.3
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7,25°C Colletotrichum graminicola
71
-
 5-hydroxymethylfurfural mutant Y334W, pH 7,25°C Colletotrichum graminicola
126
-
 5-hydroxymethylfurfural wild-type, pH 7,25°C Colletotrichum graminicola
201
-
 5-hydroxymethylfurfural mutant Y334F, pH 7,25°C Colletotrichum graminicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 substrate5-hydroxymethylfurfural Colletotrichum graminicola

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 50% of the activity at pH 7.0, substrate 5-hydroxymethylfurfural Colletotrichum graminicola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.088
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334W, pH 7,25°C Colletotrichum graminicola
0.57
-
 5-(hydroxymethyl)furan-2-carboxylic acid mutant Y334F, pH 7,25°C Colletotrichum graminicola
1.1
-
 5-(hydroxymethyl)furan-2-carboxylic acid wild-type, pH 7,25°C Colletotrichum graminicola
1.34
-
 5-hydroxymethylfurfural mutant Y334W, pH 7,25°C Colletotrichum graminicola
14.4
-
 5-hydroxymethylfurfural mutant Y334F, pH 7,25°C Colletotrichum graminicola
19.4
-
 5-hydroxymethylfurfural wild-type, pH 7,25°C Colletotrichum graminicola