Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas nitroreducens |
expression in Escherichia coli | Methylovorus sp. MP688 |
expression in Escherichia coli | Pseudomonas sp. 11/12A |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas nitroreducens | |
0.7 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas sp. 11/12A | |
0.8 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas nitroreducens | |
4.8 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas nitroreducens | |
5.1 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas nitroreducens | |
6.8 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Methylovorus sp. MP688 | |
9.1 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Methylovorus sp. MP688 | |
21.7 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas sp. 11/12A | |
38.9 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas sp. 11/12A |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylovorus sp. MP688 | E4QP00 | cf. EC 1.1.3.38 | - |
Pseudomonas nitroreducens | - |
- |
- |
Pseudomonas sp. 11/12A | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
recombinant protein, substrate vanillyl alcohol, pH 7.0, 25°C | Pseudomonas nitroreducens |
2 | - |
recombinant protein, substrate vanillyl alcohol, reaction of EC 1.1.3.47, pH 7.0, 25°C | Pseudomonas sp. 11/12A |
3.3 | - |
recombinant protein, substrate vanillyl alcohol, reaction of EC 1.1.3.38, pH 7.0, 25°C | Methylovorus sp. MP688 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-(hydroxymethyl)furfural + O2 | - |
Pseudomonas nitroreducens | furan-2,5-dicarbaldehyde + H2O2 | - |
? | |
5-(hydroxymethyl)furfural + O2 | - |
Methylovorus sp. MP688 | furan-2,5-dicarbaldehyde + H2O2 | - |
? | |
5-(hydroxymethyl)furfural + O2 | - |
Pseudomonas sp. 11/12A | furan-2,5-dicarbaldehyde + H2O2 | - |
? | |
5-formylfuran-2-carboxylate + O2 | - |
Pseudomonas nitroreducens | furan-2,5-dicarboxylate + H2O2 | - |
? | |
5-formylfuran-2-carboxylate + O2 | - |
Methylovorus sp. MP688 | furan-2,5-dicarboxylate + H2O2 | - |
? | |
5-formylfuran-2-carboxylate + O2 | - |
Pseudomonas sp. 11/12A | furan-2,5-dicarboxylate + H2O2 | - |
? | |
furan-2,5-dicarbaldehyde + O2 | - |
Pseudomonas nitroreducens | 5-formylfuran-2-carboxylate + H2O2 | - |
? | |
furan-2,5-dicarbaldehyde + O2 | - |
Methylovorus sp. MP688 | 5-formylfuran-2-carboxylate + H2O2 | - |
? | |
furan-2,5-dicarbaldehyde + O2 | - |
Pseudomonas sp. 11/12A | 5-formylfuran-2-carboxylate + H2O2 | - |
? | |
vanillyl alcohol + O2 | - |
Pseudomonas nitroreducens | vanillin + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, MALDI-TOF | Pseudomonas nitroreducens |
? | x * 57000, MALDI-TOF | Methylovorus sp. MP688 |
? | x * 57000, MALDI-TOF | Pseudomonas sp. 11/12A |
Synonyms | Comment | Organism |
---|---|---|
HMFO | - |
Pseudomonas nitroreducens |
HMFO | - |
Methylovorus sp. MP688 |
HMFO | - |
Pseudomonas sp. 11/12A |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Pseudomonas sp. 11/12A |
40 | 45 | - |
Pseudomonas nitroreducens |
45 | - |
- |
Methylovorus sp. MP688 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | more than 80% of maximum activity | Methylovorus sp. MP688 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
melting temperature | Pseudomonas sp. 11/12A |
44 | - |
melting temperature | Pseudomonas nitroreducens |
48 | - |
melting temperature | Methylovorus sp. MP688 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas sp. 11/12A | |
0.008 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas nitroreducens | |
0.01 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas sp. 11/12A | |
0.017 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas nitroreducens | |
0.1 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Methylovorus sp. MP688 | |
0.24 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas nitroreducens | |
1 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas nitroreducens | |
1 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas sp. 11/12A | |
1.38 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Methylovorus sp. MP688 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Methylovorus sp. MP688 |
7.5 | 8 | - |
Pseudomonas sp. 11/12A |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | more than 65% of maximum activity | Pseudomonas nitroreducens |
9 | - |
complete loss of activity | Pseudomonas sp. 11/12A |
10 | - |
5% residual activity | Methylovorus sp. MP688 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas nitroreducens | |
FAD | - |
Methylovorus sp. MP688 | |
FAD | - |
Pseudomonas sp. 11/12A |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methylovorus sp. MP688 | calculated from sequence | - |
6.1 |
Pseudomonas sp. 11/12A | calculated from sequence | - |
6.1 |
Pseudomonas nitroreducens | calculated from sequence | - |
6.5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Methylovorus sp. MP688 | |
0.0015 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas nitroreducens | |
0.0023 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas sp. 11/12A | |
0.0025 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas sp. 11/12A | |
0.003 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas nitroreducens | |
0.015 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Methylovorus sp. MP688 | |
0.047 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas nitroreducens | |
0.05 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas sp. 11/12A | |
0.15 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Methylovorus sp. MP688 | |
1.3 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas nitroreducens |