Literature summary for 1.1.3.47 extracted from

Daou, M.; Yassine, B.; Wikee, S.; Record, E.; Duprat, F.; Bertrand, E.; Faulds, C.
Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives (2019), Fungal Biol. Biotechnol., 6, 004 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
H2O2	2 mM, 24 h, 60% loss of activity	Trametes cinnabarina

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.18	-	furan-2,5-dicarbaldehyde	pH 6, 30°C	Trametes cinnabarina
0.21	-	furan-2,5-dicarbaldehyde	pH 6, 30°C	Trametes cinnabarina
0.61	-	5-formyl-2-furan carboxylic acid	pH 6, 30°C	Trametes cinnabarina
1.4	-	5-formyl-2-furan carboxylic acid	pH 6, 30°C	Trametes cinnabarina
5.87	-	5-(hydroxymethyl)furfural	pH 6, 30°C	Trametes cinnabarina
6.35	-	5-(hydroxymethyl)furfural	pH 6, 30°C	Trametes cinnabarina

Organism

Organism	UniProt	Comment	Textmining
Trametes cinnabarina	A0A060SXH6	isoform Glox3, cf. EC 1.2.3.15	-
Trametes cinnabarina	A0A060SYB0	cf. EC 1.2.3.15	-

Storage Stability

Storage Stability	Organism
pH 6, tartrate buffer, 48 h, 40% residual activity, stable in presence of 3 mM 5-hydroxymethylfurfural	Trametes cinnabarina

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-(hydroxymethyl)furan-2-carboxylic acid + O2	-	Trametes cinnabarina	furan-2-carbaldehyde-5-carboxylate + H2O2	-	?
5-(hydroxymethyl)furfural + O2	-	Trametes cinnabarina	furan-2,5-dicarbaldehyde + H2O2	-	?
5-(hydroxymethyl)furfural + O2	-	Trametes cinnabarina	5-(hydroxymethyl)furan-2-carboxylic acid + H2O2	-	?
furan-2,5-dicarbaldehyde + O2	-	Trametes cinnabarina	furan-2-carbaldehyde-5-carboxylate + H2O2	-	?
furan-2-carbaldehyde-5-carboxylate + O2	-	Trametes cinnabarina	furan-2,5-dicarboxylate + H2O2	-	?
additional information	major reaction product of the cascade is furan-2-carbaldehyde-5-carboxylate. The oxidation of 5-(hydroxymethyl)furfural is significantly boosted in the presence of catalase, leading to 70% yield	Trametes cinnabarina	?	-	-

Synonyms

Synonyms	Comment	Organism
Glox2	-	Trametes cinnabarina
Glox3	-	Trametes cinnabarina

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.04	-	5-formyl-2-furan carboxylic acid	pH 6, 30°C	Trametes cinnabarina
0.56	-	5-(hydroxymethyl)furfural	pH 6, 30°C	Trametes cinnabarina
0.75	-	5-(hydroxymethyl)furfural	pH 6, 30°C	Trametes cinnabarina
1.28	-	furan-2,5-dicarbaldehyde	pH 6, 30°C	Trametes cinnabarina
2	-	5-formyl-2-furan carboxylic acid	pH 6, 30°C	Trametes cinnabarina
4.8	-	furan-2,5-dicarbaldehyde	pH 6, 30°C	Trametes cinnabarina

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.072	-	5-formyl-2-furan carboxylic acid	pH 6, 30°C	Trametes cinnabarina
0.096	-	5-(hydroxymethyl)furfural	pH 6, 30°C	Trametes cinnabarina
0.118	-	5-(hydroxymethyl)furfural	pH 6, 30°C	Trametes cinnabarina
1.44	-	5-formyl-2-furan carboxylic acid	pH 6, 30°C	Trametes cinnabarina
7.268	-	furan-2,5-dicarbaldehyde	pH 6, 30°C	Trametes cinnabarina
23.4	-	furan-2,5-dicarbaldehyde	pH 6, 30°C	Trametes cinnabarina

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Release 2023.1 (January 2023)