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Literature summary for 1.1.3.6 extracted from
- Influence of long-chain primary alcohols on cholesterol oxidase from Schizophyllum commune (1983), Biochim. Biophys. Acta, 749, 69-76.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Long-chain alcohols | maximum activation with decanol and undecanol, shorter and longer alcohols are less effective | Schizophyllum commune |  |
General Stability
| General Stability | Organism |
|---|---|
| long-chain primary alcohols stabilize | Schizophyllum commune |
| SDS, 0.2 mM, stabilizes | Schizophyllum commune |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
cholesterol | - |
Schizophyllum commune | |
| 0.25 | - |
beta-sitosterol | in the presence of undecanol | Schizophyllum commune | |
| 0.45 | - |
stigmasterol | - |
Schizophyllum commune | |
| 0.5 | - |
pregnenolone | - |
Schizophyllum commune | |
| 0.5 | - |
beta-sitosterol | - |
Schizophyllum commune | |
| 1 | - |
cholesterol | in the presence of undecanol | Schizophyllum commune | |
| 1 | - |
dehydroepiandrosterone | in the presence and absence of undecanol | Schizophyllum commune | |
| 2 | - |
cholesterol | in the presence of undecanol | Schizophyllum commune | |
| 4 | - |
pregnenolone | in the presence of undecanol | Schizophyllum commune | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | Schizophyllum commune | - |
cholest-5-en-3-one + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizophyllum commune | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Schizophyllum commune |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-sitosterol + O2 | - |
Schizophyllum commune | stigmast-5-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Schizophyllum commune | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Schizophyllum commune | cholest-5-en-3-one + H2O2 | - |
? | |
| dehydroepiandrosterone + O2 | - |
Schizophyllum commune | androst-5-en-3,17-dione + H2O2 | - |
? | |
| pregnenolone + O2 | - |
Schizophyllum commune | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| stigmasterol + O2 | - |
Schizophyllum commune | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
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