Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 10 mM cyanide or 50 mM arsenite | Mycobacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Mycobacterium sp. | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | - |
probably identical with cholesterol decompositing Mycobacterium cholesterolicum | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Mycobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Mycobacterium sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Mycobacterium sp. | cholest-5-en-3-one + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
50% loss of activity after 10 min | Mycobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | strong decrease in enzyme activity above | Mycobacterium sp. |