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Literature summary for 1.1.3.6 extracted from
- Purification and characterisation of the extracellular cholesterol oxidase enzyme from Enterococcus hirae (2015), BMC Microbiol., 15, 178 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Enterococcus hirae |  |
| Co2+ | - |
Enterococcus hirae | |
| EDTA | - |
Enterococcus hirae | |
| K+ | - |
Enterococcus hirae | |
| Mg2+ | - |
Enterococcus hirae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Enterococcus hirae | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus hirae | - |
isolated from milk | - |
| Enterococcus hirae Mil-31 | - |
isolated from milk | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 2.3fold by ammnium sulfate fractionation and gel filtration | Enterococcus hirae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 124.87 | - |
purified enzyme, pH 7.5, 37°C | Enterococcus hirae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4-8°C, purified native enzyme, the enzyme maintains approximately 36% and 58.5% of its activity after 18 days of storage | Enterococcus hirae |
| 95°C, purified natie enzyme, the enzyme loses activity by gradual thermal treatment, but it maintains 58.5% activity at 95°C after 2 h | Enterococcus hirae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | - |
Enterococcus hirae | cholest-5-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Enterococcus hirae Mil-31 | cholest-5-en-3-one + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Enterococcus hirae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChOx | - |
Enterococcus hirae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Enterococcus hirae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Enterococcus hirae |
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