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Literature summary for 1.1.3.6 extracted from

  • Srivastava, A.; Singh, V.; Haque, S.; Pandey, S.; Mishra, M.; Jawed, A.; Shukla, P.K.; Singh, P.K.; Tripathi, C.K.M.
    Response surface methodology-genetic algorithm based medium optimization, purification, and characterization of cholesterol oxidase from Streptomyces rimosus (2018), Sci. Rep., 8, 10913 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.6

Inhibitors

Inhibitors Comment Organism Structure
acetone 10%, 35% loss of activity Streptomyces rimosus
Ag2+ 5 mM, 45% loss of activity Streptomyces rimosus
Benzene 10%, 20% loss of activity Streptomyces rimosus
beta-mercaptoethanol 1%, 40% loss of activity Streptomyces rimosus
chloroform 10%, 80% loss of activity Streptomyces rimosus
DMSO 10%, 11% loss of activity Streptomyces rimosus
DTT 1%, 70% loss of activity Streptomyces rimosus
Hg2+ 5 mM, 50% loss of activity Streptomyces rimosus
Pb2+ 5 mM, 35% loss of activity Streptomyces rimosus
SDS 1%, 80% loss of activity Streptomyces rimosus
Triton X-100 1%, 20% loss of activity Streptomyces rimosus
Zn2+ 5 mM, 35% loss of activity Streptomyces rimosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 cholesterol pH 7.0, 37°C Streptomyces rimosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces rimosus
-
-

Organism

Organism UniProt Comment Textmining
Streptomyces rimosus
-
-
-
Streptomyces rimosus MTCC 10792
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces rimosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cholestanol + O2 96% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
beta-cholestanol + O2 96% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?
beta-sitosterol + O2 88% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
beta-sitosterol + O2 88% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?
beta-stigmasterol + O2 68% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces rimosus cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces rimosus MTCC 10792 cholest-5-en-3-one + H2O2
-
 ?
dehydroepiandrosterone + O2 30% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
epiandrosterone + O2 15% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
ergosterol + O2 12% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
ergosterol + O2 12% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?
pregnenolone + O2 46% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
pregnenolone + O2 46% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Streptomyces rimosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 45% of maximal activity, 50°C: about 35% of maximal activity Streptomyces rimosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable at Streptomyces rimosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptomyces rimosus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 50% of maximal activity, pH 9.0: about 40% of maximal activity Streptomyces rimosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 stable at Streptomyces rimosus