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Literature summary for 1.1.3.7 extracted from

  • Vina-Gonzalez, J.; Elbl, K.; Ponte, X.; Valero, F.; Alcalde, M.
    Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution (2018), Biotechnol. Bioeng., 115, 1666-1674 .
    View publication on PubMed
Search for more literature for 1.1.3.7

Application

Application Comment Organism
synthesis expression variant carrying 4 mutations in the chimeric signal peptide (prealphaproK), plus mutations H91N/L170M in the mature protein, shows 350fold improved secretion (4.5 mg/l) in Saccharomyces cerevisiae and is stable. Expression in Pichia pastoris and fermentation in a fed-batch bioreactor enhances production to 25 mg/l. Both recombinant AAO from Saccharomyces cerevisiae and Pichia pastoris are subjected to the same N-terminal processing and have a similar pH activity profile, they differ in their kinetic parameters and thermostability Pleurotus eryngii

Protein Variants

Protein Variants Comment Organism
H91N/L170M expression variant carrying 4 mutations in the chimeric signal peptide (prealphaproK), plus mutations H91N/L170M in the mature protein, shows increased secretion upon expression in Pichia pastoris and Saccharomyces cerevisiae Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 4-methoxybenzyl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.023
-
 4-methoxybenzyl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.037
-
 4-methoxybenzyl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
0.095
-
 2,4-hexadien-1-ol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.096
-
 2,4-hexadien-1-ol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.106
-
 2,4-hexadien-1-ol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
0.34
-
 veratryl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.36
-
 veratryl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.37
-
 benzyl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.38
-
 benzyl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
0.41
-
 veratryl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
0.44
-
 benzyl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94219
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant protein from wild-type Saccharomyces cerevisiae, 60% glycosylation Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-hexadien-1-ol + 2 O2
-
 Pleurotus eryngii 2,4-hexadienal + 2 H2O2
-
 ?
4-methoxybenzyl alcohol + O2
-
 Pleurotus eryngii 4-methoxybenzyl aldehyde + H2O2
-
 ?
benzyl alcohol + O2
-
 Pleurotus eryngii benzyl aldehyde + H2O2
-
 ?
veratryl alcohol + O2
-
 Pleurotus eryngii veratryl aldehyde + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 150000, recombinant protein from wild-type Saccharomyces cerevisiae, x * 63000, recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, x * 63000, recombinant protein from wild-type Pichia pastoris Pleurotus eryngii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61.2
-
 melting temperature, recombinant protein from glycosylation-deficient Saccharomyces cerevisiae Pleurotus eryngii
61.3
-
 melting temperature, recombinant protein from wild-type Pichia pastoris Pleurotus eryngii
63
-
 melting temperature, recombinant protein from wild-type Saccharomyces cerevisiae Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.1
-
 benzyl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
23.4
-
 benzyl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
24.6
-
 veratryl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
34.4
-
 benzyl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
36.8
-
 veratryl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
41
-
 4-methoxybenzyl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
47
-
 2,4-hexadien-1-ol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
56.9
-
 4-methoxybenzyl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
56.9
-
 veratryl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
62
-
 2,4-hexadien-1-ol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
70
-
 4-methoxybenzyl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
89
-
 2,4-hexadien-1-ol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii

pI Value

Organism Comment pI Value Maximum pI Value
Pleurotus eryngii isoelelctric focusing, recombinant protein from wild-type Pichia pastoris
-
 4.5
Pleurotus eryngii isoelectric focusing, recombinant protein from glycosylation-deficient Saccharomyces cerevisiae
-
 4.6
Pleurotus eryngii isoelectric focusing, recombinant protein from wild-type Saccharomyces cerevisiae
-
 4.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
41
-
 benzyl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
62
-
 benzyl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
72
-
 veratryl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
78
-
 benzyl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
102
-
 veratryl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
139
-
 veratryl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
456
-
 2,4-hexadien-1-ol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
653
-
 2,4-hexadien-1-ol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
840
-
 2,4-hexadien-1-ol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
1782
-
 4-methoxybenzyl alcohol recombinant protein from wild-type Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii
1909
-
 4-methoxybenzyl alcohol recombinant protein from wild-type Pichia pastoris, pH 6, 25°C Pleurotus eryngii
2586
-
 4-methoxybenzyl alcohol recombinant protein from glycosylation-deficient Saccharomyces cerevisiae, pH 6, 25°C Pleurotus eryngii