Cloned (Comment) | Organism |
---|---|
expressed in Nicotiana benthamiana leaves | Arabidopsis thaliana |
expression in Nicothiana benthamiana | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-gulono-1,4-lactone | enzyme activity is inhibited when L-gulono-1,4-lactone concentration is increased to 400 mM | Arabidopsis thaliana | |
NaCl | inhibitory (20-70% residual activity) between 5 and 300 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.8 | - |
L-gulono-1,4-lactone | at pH and 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Arabidopsis thaliana | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O81030 | - |
- |
Arabidopsis thaliana | Q9LYD8 | - |
- |
Purification (Comment) | Organism |
---|---|
cation exchange cholumn chromatography and nickel affinity column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
pH 8, 25°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + O2 | - |
Arabidopsis thaliana | L-ascorbic acid + H2O2 | - |
? | |
L-gulono-1,4-lactone + cytochrome C | - |
Arabidopsis thaliana | L-ascorbate + ? | - |
? | |
additional information | no dehydrogenase or oxidase activity with L-galactono-1,4-lactone, D-galactono-1,4-lactone, and D-gluconic acid-gamma-lactone. No oxidase activity with L-gulono-1,4-lactone | Arabidopsis thaliana | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, calculated from sequence | Arabidopsis thaliana |
? | x * 65000, His6-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
At5g11540 | - |
Arabidopsis thaliana |
GulLO3 | - |
Arabidopsis thaliana |
GulLO5 | - |
Arabidopsis thaliana |
GulLO5 | isoform | Arabidopsis thaliana |
L-gulono-1,4-lactone dehydrogenase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the activity of the enzyme increases by up to 3fold when the temperature is changed from 20 to 25°C and the same is true until 35°C. At 40°C, the activity increases by at least another 1fold | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
L-gulono-1,4-lactone | at pH 8.0 and 25°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Arabidopsis thaliana |
9 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | the enzyme has a higher activity between pH 8.0 and 9.0 compared to lower pH | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | isoelectric focusing | - |
8.2 |
Arabidopsis thaliana | calculated from sequence | - |
8.2 |
General Information | Comment | Organism |
---|---|---|
physiological function | Nicotiana benthamiana leaves expressing GulLO3 do not show an increase in ascorbate content, but ascorbate content is increased in all leaves fed with L-gulono-1,4-lactone compared to water fed controls. Isoform GulLO3 protein is regulated post-transcriptionally by rapid turnover | Arabidopsis thaliana |
physiological function | Nicotiana benthamiana leaves expressing GulLO5 do not show an increase in ascorbate content | Arabidopsis thaliana |