Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.8 extracted from

  • Aboobucker, S.I.; Suza, W.P.; Lorence, A.
    Characterization of two Arabidopsis L-gulono-1,4-lactone oxidases, AtGulLO3 and AtGulLO5, involved in ascorbate biosynthesis (2017), React. Oxyg. Species (Apex), 4, 389-417 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana benthamiana leaves Arabidopsis thaliana
expression in Nicothiana benthamiana Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
L-gulono-1,4-lactone enzyme activity is inhibited when L-gulono-1,4-lactone concentration is increased to 400 mM Arabidopsis thaliana
NaCl inhibitory (20-70% residual activity) between 5 and 300 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
33.8
-
L-gulono-1,4-lactone at pH and 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Arabidopsis thaliana 5618
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O81030
-
-
Arabidopsis thaliana Q9LYD8
-
-

Purification (Commentary)

Purification (Comment) Organism
cation exchange cholumn chromatography and nickel affinity column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
pH 8, 25°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactono-1,4-lactone + O2
-
Arabidopsis thaliana L-ascorbic acid + H2O2
-
?
L-gulono-1,4-lactone + cytochrome C
-
Arabidopsis thaliana L-ascorbate + ?
-
?
additional information no dehydrogenase or oxidase activity with L-galactono-1,4-lactone, D-galactono-1,4-lactone, and D-gluconic acid-gamma-lactone. No oxidase activity with L-gulono-1,4-lactone Arabidopsis thaliana ?
-
-

Subunits

Subunits Comment Organism
? x * 65000, calculated from sequence Arabidopsis thaliana
? x * 65000, His6-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At5g11540
-
Arabidopsis thaliana
GulLO3
-
Arabidopsis thaliana
GulLO5
-
Arabidopsis thaliana
GulLO5 isoform Arabidopsis thaliana
L-gulono-1,4-lactone dehydrogenase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 the activity of the enzyme increases by up to 3fold when the temperature is changed from 20 to 25°C and the same is true until 35°C. At 40°C, the activity increases by at least another 1fold Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
L-gulono-1,4-lactone at pH 8.0 and 25°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Arabidopsis thaliana
9
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
8 9 the enzyme has a higher activity between pH 8.0 and 9.0 compared to lower pH Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana isoelectric focusing
-
8.2
Arabidopsis thaliana calculated from sequence
-
8.2

General Information

General Information Comment Organism
physiological function Nicotiana benthamiana leaves expressing GulLO3 do not show an increase in ascorbate content, but ascorbate content is increased in all leaves fed with L-gulono-1,4-lactone compared to water fed controls. Isoform GulLO3 protein is regulated post-transcriptionally by rapid turnover Arabidopsis thaliana
physiological function Nicotiana benthamiana leaves expressing GulLO5 do not show an increase in ascorbate content Arabidopsis thaliana