BRENDA - Enzyme Database show
show all sequences of 1.1.5.12

Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate

Kato, O.; Youn, J.; Stansen, K.; Matsui, D.; Oikawa, T.; Wendisch, V.; BMC Microbiol. 10, 321 (2010)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.61
-
D-lactate
pH 7.5, 30°C
Corynebacterium glutamicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum ATCC 13032
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + 2,6-dichloroindophenol
-
727253
Corynebacterium glutamicum
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
D-lactate + 2,6-dichloroindophenol
-
727253
Corynebacterium glutamicum ATCC 13032
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
DL-2-hydroxybutanoate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum
2-oxobutanoate + reduced 2,6-dichloroindophenol
-
-
-
?
DL-2-hydroxybutanoate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum ATCC 13032
2-oxobutanoate + reduced 2,6-dichloroindophenol
-
-
-
?
L-lactate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
L-lactate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum ATCC 13032
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
additional information
no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate
727253
Corynebacterium glutamicum
?
-
-
-
-
additional information
no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate
727253
Corynebacterium glutamicum ATCC 13032
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Corynebacterium glutamicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Corynebacterium glutamicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.61
-
D-lactate
pH 7.5, 30°C
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + 2,6-dichloroindophenol
-
727253
Corynebacterium glutamicum
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
D-lactate + 2,6-dichloroindophenol
-
727253
Corynebacterium glutamicum ATCC 13032
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
DL-2-hydroxybutanoate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum
2-oxobutanoate + reduced 2,6-dichloroindophenol
-
-
-
?
DL-2-hydroxybutanoate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum ATCC 13032
2-oxobutanoate + reduced 2,6-dichloroindophenol
-
-
-
?
L-lactate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
L-lactate + 2,6-dichloroindophenol
about 5% of the activity with D-lactate
727253
Corynebacterium glutamicum ATCC 13032
pyruvate + reduced 2,6-dichloroindophenol
-
-
-
?
additional information
no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate
727253
Corynebacterium glutamicum
?
-
-
-
-
additional information
no significant reaction with D-malate, L-malate, D-tartrate and L-tartrate
727253
Corynebacterium glutamicum ATCC 13032
?
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
inactivation of Dld results in the loss of the ability to grow with D-lactate. Heterologous expression in Corynebacterium efficiens enables this species to grow with D-lactate as sole carbon source
Corynebacterium glutamicum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
inactivation of Dld results in the loss of the ability to grow with D-lactate. Heterologous expression in Corynebacterium efficiens enables this species to grow with D-lactate as sole carbon source
Corynebacterium glutamicum
Other publictions for EC 1.1.5.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727253
Kato
Quinone-dependent D-lactate de ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
BMC Microbiol.
10
321
2010
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1
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25
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8
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1
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1
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1
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8
-
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1
1
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-
740031
Peersen
Site-specific incorporation of ...
Escherichia coli
Biochemistry
29
3256-3262
1990
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1
22
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8
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1
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1
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1
22
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8
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1
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740030
Matsushita
D-Lactate oxidation and genera ...
Escherichia coli, Escherichia coli GR19N
Biochemistry
25
2321-2327
1986
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3
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1
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4
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1
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4
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-
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1
1
-
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-
740029
Pratt
Membrane-bound D-lactate dehyd ...
Escherichia coli, Escherichia coli W3110trpA33
Biochemistry
18
312-316
1979
1
-
-
-
-
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1
1
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1
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2
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1
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1
2
2
1
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
2
2
1
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1
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1
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740028
Futai
Membrane D-lactate dehydrogena ...
Escherichia coli
Biochemistry
12
2468-2474
1973
1
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-
-
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1
4
1
-
2
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1
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1
-
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-
-
2
3
1
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-
-
-
1
-
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1
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-
1
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1
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1
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4
1
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2
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1
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2
3
1
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1
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-
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740643
Kohn
Mechanisms of active transport ...
Escherichia coli, Escherichia coli ML 308-225
J. Biol. Chem.
248
7012-7017
1973
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-
-
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-
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2
4
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1
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3
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1
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8
1
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-
2
-
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1
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1
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2
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4
-
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1
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1
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8
1
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2
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