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Literature summary for 1.1.5.12 extracted from
- Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties (1973), Biochemistry, 12, 2468-2474.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH. At 1.5 mM, 50% inhibition at pH 9.3 | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
(R)-lactate | pH 8.0, 23°C | Escherichia coli | |
| 1.2 | - |
(R,S)-2-hydroxybutanoate | pH 8.0, 23°C | Escherichia coli | |
| 1.4 | - |
(R)-lactate | presence of cyanide, pH 8.0, 23°C | Escherichia coli | |
| 18 | - |
(S)-lactate | pH 8.0, 23°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
sucrose density gradient centrifugation | Escherichia coli |
| 72000 | - |
1 * 72000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C, 20% ethylene glycol, stable for at least 2 weeks | Escherichia coli |
| 0°C, 5 days, 80% residual activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + phenazine methosulfate | - |
Escherichia coli | pyruvate + reduced phenazine methosulfate | - |
? | |
| (R,S)-2-hydroxybutanoate + a quinone | 12% of the activity with (R)-lactate | Escherichia coli | 2-oxobutanoate + a quinol | - |
? | |
| (S)-lactate + oxidized dichlorophenolindophenol | 14% of the activity with (R)-lactate | Escherichia coli | pyruvate + reduced dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 72000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Escherichia coli | |
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