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show all sequences of 1.1.5.12

D-Lactate oxidation and generation of the proton electrochemical gradient in membrane vesicles from Escherichia coli GR19N and in proteoliposomes reconstituted with purified D-lactate dehydrogenase and cytochrome O oxidase

Matsushita, K., Kaback, H.R.; Biochemistry 25, 2321-2327 (1986)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli GR19N
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + ferricyanide
-
740030
Escherichia coli
pyruvate + ferrocyanide
-
-
-
?
D-lactate + ferricyanide
-
740030
Escherichia coli GR19N
pyruvate + ferrocyanide
-
-
-
?
D-lactate + ubiquinone 1
-
740030
Escherichia coli
pyruvate + reduced ubiquinone 1
-
-
-
?
D-lactate + ubiquinone 1
-
740030
Escherichia coli GR19N
pyruvate + reduced ubiquinone 1
-
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + ferricyanide
-
740030
Escherichia coli
pyruvate + ferrocyanide
-
-
-
?
D-lactate + ferricyanide
-
740030
Escherichia coli GR19N
pyruvate + ferrocyanide
-
-
-
?
D-lactate + ubiquinone 1
-
740030
Escherichia coli
pyruvate + reduced ubiquinone 1
-
-
-
?
D-lactate + ubiquinone 1
-
740030
Escherichia coli GR19N
pyruvate + reduced ubiquinone 1
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
D-lactate/ubiquinone 1 or D-lactate/ferricyanide oxidoreductase activity does not generate a membranepotential, suggesting that electron flow from D-lactate dehydrogenase to ubiquinone is not electrogenic. Proteoliposomes reconstituted with purified D-lactate dehydrogenase, ubiquinone 8, and purified cytochrome o catalyze D-lactate and ubiquinol 1 oxidation and generate a H+ electrochemical gradient similar to that observed in membrane vesicles. The only component between D-lactate dehydrogenase or ubiquinol and oxygen in the membranes that is directly involved in the generation of the H+ electrochemical gradient is cytochrome 0
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
D-lactate/ubiquinone 1 or D-lactate/ferricyanide oxidoreductase activity does not generate a membranepotential, suggesting that electron flow from D-lactate dehydrogenase to ubiquinone is not electrogenic. Proteoliposomes reconstituted with purified D-lactate dehydrogenase, ubiquinone 8, and purified cytochrome o catalyze D-lactate and ubiquinol 1 oxidation and generate a H+ electrochemical gradient similar to that observed in membrane vesicles. The only component between D-lactate dehydrogenase or ubiquinol and oxygen in the membranes that is directly involved in the generation of the H+ electrochemical gradient is cytochrome 0
Escherichia coli
Other publictions for EC 1.1.5.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727253
Kato
Quinone-dependent D-lactate de ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
BMC Microbiol.
10
321
2010
-
-
-
-
-
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1
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-
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-
25
-
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8
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1
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1
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1
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8
-
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-
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-
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1
1
-
-
-
740031
Peersen
Site-specific incorporation of ...
Escherichia coli
Biochemistry
29
3256-3262
1990
-
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-
1
22
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8
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1
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1
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1
22
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8
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1
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-
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-
-
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740030
Matsushita
D-Lactate oxidation and genera ...
Escherichia coli, Escherichia coli GR19N
Biochemistry
25
2321-2327
1986
-
-
-
-
-
-
-
-
-
-
-
-
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3
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1
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4
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-
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-
-
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-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
740029
Pratt
Membrane-bound D-lactate dehyd ...
Escherichia coli, Escherichia coli W3110trpA33
Biochemistry
18
312-316
1979
1
-
-
-
-
-
-
1
1
-
1
-
-
2
-
-
1
-
-
-
1
2
2
1
-
-
1
-
1
-
-
1
-
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-
1
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-
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
1
2
2
1
-
-
1
-
1
-
-
-
-
-
-
-
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740028
Futai
Membrane D-lactate dehydrogena ...
Escherichia coli
Biochemistry
12
2468-2474
1973
1
-
-
-
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-
1
4
1
-
2
-
-
1
-
-
1
-
-
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-
2
3
1
-
-
-
-
1
-
-
1
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-
1
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1
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-
-
-
1
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4
1
-
2
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-
1
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-
2
3
1
-
-
-
-
1
-
-
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-
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740643
Kohn
Mechanisms of active transport ...
Escherichia coli, Escherichia coli ML 308-225
J. Biol. Chem.
248
7012-7017
1973
-
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-
-
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2
4
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1
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3
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1
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8
1
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2
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1
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1
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2
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4
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1
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1
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8
1
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2
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